Papers

Peer-reviewed
7 2016

Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Tatsuya Hori
  • ,
  • Hitoshi Ushijima
  • ,
  • Taku Kimura
  • ,
  • Masanori Kobayashi
  • ,
  • Eiichi Kawakami
  • ,
  • Toshihiko Tsutsui

Volume
78
Number
7
First page
1137
Last page
1143
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1292/jvms.16-0037
Publisher
JAPAN SOC VET SCI

Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow freezing method was judged to be normal, but no conception occurred in any of the recipient bitches. Two of the five bitches that received transferred embryos (morula to early blastocyst stages) vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9 embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was more appropriate for canine embryo cryopreservation than the slow-freezing method, which is used for the cryopreservation of embryos of other mammalian species.

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Link information
DOI
https://doi.org/10.1292/jvms.16-0037
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27041356
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000380622000007&DestApp=WOS_CPL