論文

査読有り
2016年7月

Intrauterine embryo transfer with canine embryos cryopreserved by the slow freezing and the Cryotop method

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Tatsuya Hori
  • ,
  • Hitoshi Ushijima
  • ,
  • Taku Kimura
  • ,
  • Masanori Kobayashi
  • ,
  • Eiichi Kawakami
  • ,
  • Toshihiko Tsutsui

78
7
開始ページ
1137
終了ページ
1143
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.16-0037
出版者・発行元
JAPAN SOC VET SCI

Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow freezing method was judged to be normal, but no conception occurred in any of the recipient bitches. Two of the five bitches that received transferred embryos (morula to early blastocyst stages) vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9 embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was more appropriate for canine embryo cryopreservation than the slow-freezing method, which is used for the cryopreservation of embryos of other mammalian species.


リンク情報
DOI
https://doi.org/10.1292/jvms.16-0037
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27041356
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000380622000007&DestApp=WOS_CPL