2020年5月
RNA sequence analysis of growth-related genes in penaeus monodon
Walailak Journal of Science and Technology
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- 巻
- 17
- 号
- 5
- 開始ページ
- 489
- 終了ページ
- 502
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
Penaeus monodon is one of the most economically important shrimp species in Thailand. However, little information is available about the functional genomics related to its growth performance. In this study, Illumina paired-end sequencing was used to analyze transcriptomes related to growth performance in P. monodon muscle. A total of 38.4 million reads were generated. The pooled reads, from 10 libraries, were de novo assembled into 113,991 genes, with an average length of 337 bp. Gene expression was analyzed with the edgeR program, which revealed 705 differentially expressed contigs (p < 0.05) in fastgrowth shrimp compared to slow-growth shrimp. The results show the 234 up-regulated contigs in fastgrowth shrimp are mostly underlined genes involving the metabolic pathway. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed seven genes involved in the cell cycle that were expressed more in fast-growth shrimp (p < 0.05) than in slow-growth shrimp, and moderately to strongly correlated with shrimp body weight. These genes may be good candidates for growth performance improvement in P. monodon.
- リンク情報
- ID情報
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- ISSN : 1686-3933
- eISSN : 2228-835X
- SCOPUS ID : 85084842786