Tetsuro Kokubo

Profile Information

Affiliation: School of Science, Graduate School of Medical Life Science Department of Medical Life Science, Yokohama City University

Degree: 農学博士(東京大学)

J-GLOBAL ID: 200901025817184333
researchmap Member ID: 1000193401

External link: http://www.tsurumi.yokohama-cu.ac.jp/mcbl/index2.html

1985年東京大学農学部農芸化学科卒業
1990年東京大学大学院農学系研究科博士課程修了
1990年12月-1995年3月アメリカ合衆国国立衛生研究所（NIH）研究員
1995年4月-2001年3月奈良先端科学技術大学院大学助教授
2001年4月- 横浜市立大学教授

Research Interests

Research Areas

Life sciences / Molecular biology /

Research History

Professor, Yokohama City University International College of Arts and Sciences Medical Life Science Graduate School of Medical Life Science Department of Medical Life Science

Education

1985 - 1990

The University of Tokyo
- 1985

The University of Tokyo
- 1985

Faculty of Agriculture, The University of Tokyo

Awards

2000

NAIST学術賞

Papers

Taf1 N-terminal domain 2 (TAND2) of TFIID promotes formation of stable and mobile unstable TBP-TATA complexes

Shinji Miyasaka, Ryota Kitada, Tetsuro Kokubo

Gene, 889 147800-147800, Dec, 2023 Peer-reviewedLast authorCorresponding author
TFIID dependency of steady-state mRNA transcription altered epigenetically by simultaneous functional loss of Taf1 and Spt3 is Hsp104-dependent

Ryo Iwami, Naoki Takai, Minenosuke Matsutani, Yuh Shiwa, Haruki Kokubo, Koji Kasahara, Tetsuro Kokubo

PLoS ONE, 18(2) e0281233, Feb 9, 2023 Peer-reviewedLast authorCorresponding author
The function of Spt3, a subunit of the SAGA complex, in PGK1 transcription is restored only partially when reintroduced by plasmid into taf1 spt3 double mutant yeast strains

Ryo Iwami, Naoki Takai, Tetsuro Kokubo

Genes & Genetic Systems, 95(3) 151-163, Aug 27, 2020 Peer-reviewed
Fpr1, a primary target of rapamycin, functions as a transcription factor for ribosomal protein genes cooperatively with Hmo1 in Saccharomyces cerevisiae

Koji Kasahara, Risa Nakayama, Yuh Shiwa, Yu Kanesaki, Taichiro Ishige, Hirofumi Yoshikawa, Tetsuro Kokubo

PLOS Genetics, 16(6) e1008865-e1008865, Jun 30, 2020 Peer-reviewed
Multiple direct interactions of TBP with the MYC oncoprotein

Yong Wei, Diana Resetca, Zhe Li, Isak Johansson-Åkhe, Alexandra Ahlner, Sara Helander, Amelie Wallenhammar, Vivian Morad, Brian Raught, Björn Wallner …

Nature Structural & Molecular Biology, 26(11) 1035-1043, Nov, 2019 Peer-reviewed
Transcriptional activation is weakened when Taf1p N-terminal domain 1 is substituted with its Drosophila counterpart in yeast TFIID

Koji Kasahara, Shinya Takahata, Tetsuro Kokubo

Genes & Genetic Systems, 94(1) 51-59, Feb 1, 2019 Peer-reviewed
SAGA mediates transcription from the TATA-like element independently of Taf1p/TFIID but dependent on core promoter structures in Saccharomyces cerevisiae

Kiyoshi Watanabe, Tetsuro Kokubo

PLOS ONE, 12(11) e0188435, Nov, 2017 Peer-reviewed
Oligomerization of Hmo1 mediated by box A is essential for DNA binding in vitro and in vivo

Koji Kasahara, Ayako Higashino, Satoru Unzai, Hirofumi Yoshikawa, Tetsuro Kokubo

GENES TO CELLS, 21(12) 1333-1352, Dec, 2016 Peer-reviewed
A Random Screen Using a Novel Reporter Assay System Reveals a Set of Sequences That Are Preferred as the TATA or TATA-Like Elements in the CYC1 Promoter of Saccharomyces cerevisiae

Kiyoshi Watanabe, Makoto Yabe, Koji Kasahara, Tetsuro Kokubo

PLOS ONE, 10(6) e0129357, Jun, 2015 Peer-reviewed
A Novel Quantitative Hemolytic Assay Coupled with Restriction Fragment Length Polymorphisms Analysis Enabled Early Diagnosis of Atypical Hemolytic Uremic Syndrome and Identified Unique Predisposing Mutations in Japan

Yoko Yoshida, Toshiyuki Miyata, Masanori Matsumoto, Hiroko Shirotani-Ikejima, Yumiko Uchida, Yoshifumi Ohyama, Tetsuro Kokubo, Yoshihiro Fujimura

PLOS ONE, 10(5) e0124655-e0124655, May 7, 2015 Peer-reviewed
Both HMG boxes in Hmo1 are essential for DNA binding in vitro and in vivo

Ayako Higashino, Yuh Shiwa, Hirofumi Yoshikawa, Tetsuro Kokubo, Koji Kasahara

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 79(3) 384-393, Mar, 2015 Peer-reviewed
The physical size of transcription factors is key to transcriptional regulation in chromatin domains

Kazuhiro Maeshima, Kazunari Kaizu, Sachiko Tamura, Tadasu Nozaki, Tetsuro Kokubo, Koichi Takahashi

JOURNAL OF PHYSICS-CONDENSED MATTER, 27(6) 064116, Feb, 2015 Peer-reviewed
High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation

Madhanagopal Anandapadamanaban, Cecilia Andresen, Sara Helander, Yoshifumi Ohyama, Marina I. Siponen, Patrik Lundstrom, Tetsuro Kokubo, Mitsuhiko Ikura, Martin Moche, Maria Sunnerhagen

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 20(8) 1008-+, Aug, 2013 Peer-reviewed
Identification and Recombinant Analysis of Botrocetin-2, a Snake Venom Cofactor for von Willebrand Factor-Induced Platelet Agglutination

Yukiyo Yamamoto-Suzuki, Yoshihiko Sakurai, Yoshihiro Fujimura, Masanori Matsumoto, Jiharu Hamako, Tetsuro Kokubo, Hitoshi Kitagawa, Sarkar M. A. Kawsar, Yuki Fujii, Yasuhiro Ozeki …

BIOCHEMISTRY, 51(26) 5329-5338, Jul, 2012 Peer-reviewed
Rapid cell division contributes to efficient induction of A/T mutations during Ig gene hypermutation

Chie Kano, Rika Ouchida, Tetsuro Kokubo, Ji-Yang Wang

MOLECULAR IMMUNOLOGY, 48(15-16) 1993-1999, Sep, 2011 Peer-reviewed
Cell Polarity in Saccharomyces cerevisiae Depends on Proper Localization of the Bud9 Landmark Protein by the EKC/KEOPS Complex

Yu Kato, Hiroshi Kawasaki, Yoshifumi Ohyama, Takashi Morishita, Hiroshi Iwasaki, Tetsuro Kokubo, Hisashi Hirano

GENETICS, 188(4) 871-U214, Aug, 2011 Peer-reviewed
Hmo1 directs pre-initiation complex assembly to an appropriate site on its target gene promoters by masking a nucleosome-free region

Koji Kasahara, Yoshifumi Ohyama, Tetsuro Kokubo

NUCLEIC ACIDS RESEARCH, 39(10) 4136-4150, May, 2011 Peer-reviewed
Highly redundant function of multiple AT-rich sequences as core promoter elements in the TATA-less RPS5 promoter of Saccharomyces cerevisiae

Fuminori Sugihara, Koji Kasahara, Tetsuro Kokubo

NUCLEIC ACIDS RESEARCH, 39(1) 59-75, Jan, 2011 Peer-reviewed
Saccharomyces cerevisiae Ssd1p promotes CLN2 expression by binding to the 5 '-untranslated region of CLN2 mRNA

Yoshifumi Ohyama, Koji Kasahara, Tetsuro Kokubo

GENES TO CELLS, 15(12) 1169-1188, Dec, 2010 Peer-reviewed
Saccharomyces cerevisiae Ssd1p promotes CLN2 expression by binding to the 5'-untranslated region of CLN2 mRNA

Yoshifumi Ohyama, Koji Kasahara, Tetsuro Kokubo

Genes to Cells, 15(12) 1169-1188, Dec, 2010 Peer-reviewed

Misc.

Regulatory mechanism for transcription of ribosomal protein genes in Candida glabrata.

中村美春, 島村直希, 竹内建樹, 中林幸佳, 志波優, 松谷峰之介, 古久保哲朗, 知花博治, 笠原浩司

日本分子生物学会年会プログラム・要旨集(Web), 46th, 2023
出芽酵母のFK506/ラパマイシン結合タンパク質Fpr1は,リボソームタンパク質遺伝子の転写因子として働く

笠原浩司, 中山理紗, 志波優, 兼崎友, 石毛太一郎, 吉川博文, 古久保哲朗

日本分子生物学会年会プログラム・要旨集(Web), 41st, 2018
Atypical Hemolytic Uremic Syndrome In Japan Characterized By The Inhibitory Antibody-Based Hemolytic Assay and The Gene Analysis

Yoko Yoshida, Xinping Fan, Yoshifumi Ohyama, Tetsuro Kokubo, Masanori Matsumoto, Hideo Yagi, Hiroko Shirotani-Ikejima, Toshiyuki Miyata, Yoshihiro Fujimura

BLOOD, 122(21), Nov, 2013
本邦の非典型溶血性尿毒症症候群(aHUS)の患者登録とその表現型-遺伝子型解析

吉田瑶子, 範新萍, 本田繁則, 芦田明, 服部元史, 松本雅則, 大山良文, 古久保哲朗, 南学正臣, 宮田敏行 …

日本小児腎臓病学会雑誌, 26(1Suppl.) 143-143, May, 2013
FRET（蛍光共鳴エネルギー移動）分光法～タンパク質間相互作用の解析～

奇世媛, Tapas K Mal、Le Zheng, 伊倉光彦, 古久保哲朗

実験医学別冊：生命科学のための機器分析実験ハンドブック, 35-40, 2007 Invited
V章遺伝子発現系 5.1 転写

古久保哲朗

酵母のすべて ◎系統, 細胞から分子まで, 119-126, 2007 Invited
磁気共鳴法を利用した生細胞での遺伝子発現解析

古久保哲朗, 奇世媛, 杉原文徳, 白川昌宏

バイオテクノロジージャーナル, 6(5) 605-608, 2006 Invited
第1章転写開始反応の制御機構

古久保哲朗

バイオ研究マスターシリーズ転写研究集中マスター, 42-51, 2005 Invited
第５章転写開始・伸長反応の制御機構

古久保哲朗

バイオサイエンスの新世紀第１巻遺伝子の構造と機能, 51-64, 2001 Invited
転写調節因子群と転写調節反応機構論

古久保哲朗

遺伝子発現〜ジーンセレクターから生命現象へ〜, 87-105, 2001 Invited
基本転写因子TFIIDサブユニット(TAFs)の機能解析

古久保哲朗

蛋白質核酸酵素増刊号「転写因子の機能-転写制御複合体形成のダイナミクス-」, 45(9) 1484-1493, 2000 Invited
The cDNA cloning of human placental ecto-ATP diphosphohydrolase (ATPDase).

M Matsumoto, Y Sakurai, T Kokubo, Yagi, N Narita, Y Ikeda, K Titani, Y Fujimura

BLOOD, 92(10) 78B-78B, Nov, 1998
TATAボックス構造をまねる転写因子-TAF230のユニークな転写阻害機構-

中谷喜洋, 古久保哲朗, 伊倉光彦

実験医学, 16(19) 2474-2476, 1998
Dimeric structure of von Willebrand factor A1-loop region is required to show a complete inhibition on high-shear stress induced platelet aggregation.

S Miura, Y Sakurai, H Takatsuka, A Yoshioka, H Yagi, T Kokubo, M Kitoh, Y Ikeda, T Matsui, K Titani …

BLOOD, 90(10) 2070-2070, Nov, 1997
Isolation and cloning of mamushigin, a novel platelet glycoprotein Ib binding protein from Agkistrodon halys bromhoffii

Y Sakurai, Y Fujimura, K Titani, T Matsui, M Suzuki, T Kawasaki, T Kokubo, K Imamura, M Kawaichi, A Yoshioka

THROMBOSIS AND HAEMOSTASIS, OC734-OC734, Jun, 1997
基本転写因子TFIIDの構造と機能

古久保哲朗

日本血栓止血学会誌, 7(4) 257-267, 1996 Invited
転写因子：基礎から臨床へ

橘正芳, 古久保哲朗

Molecular Medicine, 32(5) 468-476, 1995 Invited
転写因子概説

古久保哲朗

Molecular Medicine, 32(5) 478-486, 1995 Invited

Books and Other Publications

Mechanisms of Transcriptional Activation and Repression

KOKUBO Tetsuro

Springer Science + Business Media LLC, 2013
Transcription Initiation in Eukaryote

Tetsuro Kokubo

Springer Science + Business Media, 2013
Transcription in Eukaryote

Tetsuro Kokubo

Springer Science + Business Media, 2013

Presentations

出芽酵母においてTAF1のN末端領域欠失変異はRAMシグナル経路の各種変異と合成致死性を示す

第32回日本分子生物学会年会, 2009
Deletion of the N-terminal domain of TAF1 (taf1-ΔTAND) exhibits synthetic lethality with mutations of the RAM signaling network in budding yeast

2009
リボソームタンパク質遺伝子のプロモーターにおけるHMO1の分子機能

内藤コンファレンス／Nuclear Dynamics and RNA [I], 2008
TFIIDサブユニットのゲノムワイドなプロモーター結合解析

第31回日本分子生物学会年会・第81回日本生化学会大会合同大会, 2008
出芽酵母BEM4に見出された転写因子としての新たな機能

第31回日本分子生物学会年会・第81回日本生化学会大会合同大会, 2008
出芽酵母のHMGBファミリータンパク質HMO1によるリボソームタンパク質遺伝子の転写開始点決定機構

第31回日本分子生物学会年会・第81回日本生化学会大会合同大会, 2008
Molecular function of HMO1 at ribosomal protein gene promoters

2008
出芽酵母のrRNA遺伝子及びリボソームタンパク質遺伝子群における転写調節因子の集合

2007
ASSEMBLY OF REGULATORY FACTORS ON rRNA and RIBOSOMAL PROTEIN GENES IN S. CEREVISIAE

MECHANISMS OF EUKARYOTIC TRANSCRIPTION, 2007
CLN2遺伝子発現における多形質性因子SSD1の分子機能

日本分子生物学会2007フォーラム, 2006
出芽酵母Med9の欠失変異体解析による機能ドメインの同定

日本分子生物学会2006フォーラム, 2006
A role of the polymorphic gene SSD1 in the expression of CLN2 in Saccharomyces cerevisiae

2006
Identification and characterization of functional domains of Med9 in Saccharomyces cerevisiae

2006

Professional Memberships

Research Projects

Functional analysis of liquid droplet formed by coupling of specific RNA binding proteins and a basal transcription machinery

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2018 - Mar, 2021

Kokubo Tetsuro
Mechanism of how core transcription factors are involved in regulating transcription and post-transcriptional mRNA fate

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2015 - Mar, 2018

KOKUBO TETSURO
基本転写因子TFIIDを介した転写調節機構の解明

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, Apr, 2013 - Mar, 2015

古久保哲朗
Mechanism of how core transcription factors affect the fate of mRNA

Grants-in-Aid for Scientific Research, Grant-in-Aid for Challenging Exploratory Research, Japan Society for the Promotion of Science, Apr, 2012 - Mar, 2015

KOKUBO Tetsuro
Mechanisms of core promoter recognition by general transcription factor TFIID

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, Apr, 2011 - Mar, 2014

KOKUBO Tetsuro
基本転写因子TFIIDの機能発現における天然変性領域の役割

科学研究費助成事業, 新学術領域研究(研究領域提案型), 日本学術振興会, 2010 - 2011

古久保哲朗
Mechanisms of transcriptional initiation and regulation mediated by general transcription factor TFIID

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2008 - 2010

KOKUBO Tetsuro
転写制御における基本転写因子TFIIDサブユニット(TAFs)の分子機能

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2008 - 2009

古久保哲朗
Studies on the regulatory mechanisms of TFIID-mediated eukaryotic transcription

Grants-in-Aid for Scientific Research, Grant-in-Aid for Scientific Research (B), Japan Society for the Promotion of Science, 2006 - 2007

KOKUBO Tetsuro
転写制御における基本転写因子TFIIDサブユニット(TAFs)の分子機能

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2006 - 2007

古久保哲朗
転写制御における基本転写因子TFIIDサブユニット(TAFs)の分子機能

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2005 - 2005

古久保哲朗
新規in vivo可視化技術を用いた真核細胞における遺伝情報発現機構の解析

科学研究費助成事業, 学術創成研究費, 日本学術振興会, 2001 - 2005

古久保哲朗, 白川昌宏
基本転写因子TFIIDに支配される標的遺伝子群の網羅的単離とそのプロモーター特性

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2004 - 2004

古久保哲朗
基本転写因子TFIIDに支配される標的遺伝子群の網羅的単離とそのプロモーター特性

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2003 - 2003

古久保哲朗
基本転写因子TFIIDに支配される標的遺伝子群の網羅的単離とそのプロモーター特性

科学研究費助成事業, 特定領域研究, 日本学術振興会, 2002 - 2002

古久保哲朗
転写調節における基本転写因子TFIIDサブユニット(TAFs)の分子機能

科学研究費助成事業, 基盤研究(C), 日本学術振興会, 2001 - 2001

古久保哲朗
基本転写因子TFIIDに支配される標的遺伝子群の網羅的単離とそのプロモーター特性

科学研究費助成事業, 特定領域研究(C), 日本学術振興会, 2001 - 2001

古久保哲朗
標的分子であるTBPに結合した転写活性化ドメインの立体構造解析

科学研究費助成事業, 特定領域研究(A), 日本学術振興会, 2001 - 2001

古久保哲朗
基本転写因子TFIIDに支配される標的遺伝子群の網羅的単離とそのプロモーター特性

科学研究費助成事業, 特定領域研究(C), 日本学術振興会, 2000 - 2000

古久保哲朗
標的分子であるTBPに結合した転写活性化ドメインの立体構造解析

科学研究費助成事業, 特定領域研究(A), 日本学術振興会, 2000 - 2000

古久保哲朗

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Education

Awards

Papers

Misc.

Books and Other Publications

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