MISC

1996年9月

Diagnosis of leprosy by the practical application of the polymerase chain reaction

EUROPEAN JOURNAL OF DERMATOLOGY
  • Y Sugita
  • ,
  • M Miyamoto
  • ,
  • M Koseki
  • ,
  • N Ishii
  • ,
  • H Nakajima

6
6
開始ページ
423
終了ページ
426
記述言語
英語
掲載種別
出版者・発行元
JOHN LIBBEY EUROTEXT LTD

We have developed a practical method of detecting Mycobacterium leprae DNA in leprosy patients using the polymerase chain reaction (PCR). We have designed a new set of primer oligonucleotides based on the DNA sequence encoding the M. leprae heat shock protein (hsp) 70. In order to avoid non-specific amplification of human or other bacterial DNA, regions of hsp 70 with low amino acid sequence homologies were selected for PCR primers, which also showed low homologies at the nucleic acid level. Instead of skin biopsy specimens, tissue fluid, which may contain M. leprae, was obtained from a needle inserted in the skin. The specificity of the amplified DNA fragment was confirmed by a simplified detection method, which produced the same results as Southern blot analysis. This study demonstrated useful, practical improvements of the PCR method that enable leprosy patients to be detected in the field.

Web of Science ® 被引用回数 : 3

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996VF74900006&DestApp=WOS_CPL

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