TEZUKA Kenichi

J-GLOBAL         Last updated: Dec 11, 2019 at 02:48
 
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Name
TEZUKA Kenichi
Affiliation
Gifu University
Section
Graduate School of Medicine
Degree
(BLANK)(Meikai University)

Research Areas

 
 

Academic & Professional Experience

 
 
   
 
Associate Professor, Gifu University Graduate School of Medicine
 
Apr 2002
 - 
Mar 2007
Associate Professor, Gifu University Graduate School of Medicine
 
1997
 - 
2001
Science Univ. of Tokyo,
 
1994
 - 
1997
Merck Sharp & Dhome,
 
1991
 - 
1994
Meikai University, School of Dentistry
 

Education

 
 
 - 
1987
Faculty of Science, Kyoto University
 

Awards & Honors

 
2012
J. Gies Award-Biological Research, IADA/AADA
 

Published Papers

 
FGF2‐responsive genes in human dental pulp cells assessed using a rat spinal cord injury model .
Ken Sugiyama, Kosuke Nagashima, Takahiro Miwa, Yuta Shimizu, Tomoko Kawaguchi, Kazuki Iida, Naritaka Tamaoki, Daijiro Hatakeyama, Hitomi Aoki, Chikara Abe, Hironobu Morita, Takahiro Kunisada, Toshiyuki Shibata, Hidefumi Fukumitsu, Ken-ichi Tezuka
J. Bone Miner. Metabol   37 467-474   2019   [Refereed]
Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury.
Kosuke Nagashima, Takahiro Miwa, Hitomi Soumiya, Daisuke Ushiro, Tomoko Takeda-Kawaguchi, Naritaka Tamaoki, Saho Ishiguro, Yumi Sato, Kei Miyamoto, Takatoshi Ohno, Masatake Osawa, Takahiro Kunisada, Toshiyuki Shibata, Ken-ichi Tezuka, Shoei Furukawa and Hidefumi Fukumitsu
Sci. Rep.   7 13500   2017   [Refereed]
Naritaka Tamaoki, Kazutoshi Takahashi, Hitomi Aoki, Kazuki Iida, Tomoko Kawaguchi, Daijirou Hatakeyama, Masatoshi Inden, Naoyuki Chosa, Akira Ishisaki, Takahiro Kunisada, Toshiyuki Shibata, Naoki Goshima, Shinya Yamanaka, Ken Ichi Tezuka
Scientific Reports   4    Jan 2014
The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by defined transcription factors has been a well-established technique and will provide an invaluable resource for regenerative medicine. However, the low reprogramming...
K. Iida, T. Kawaguchi, M. Hada, M. Yuriguchi, H. Aoki, N. Tamaoki, D. Hatakeyama, T. Kunisada, T. Shibata, and K. Tezuka
J. Dental Res.   92 905-910   2013   [Refereed]
Keisuke Okita, Yasuko Matsumura, Yoshiko Sato, Aki Okada, Asuka Morizane, Satoshi Okamoto, Hyenjong Hong, Masato Nakagawa, Koji Tanabe, Ken-ichi Tezuka, Toshiyuki Shibata, Takahiro Kunisada, Masayo Takahashi, Jun Takahashi, Hiroh Saji, and Shinya Yamanaka
Nature Methods.   8 409-412   2011   [Refereed]
Kazuki Iida, Tomoko Takeda-Kawaguchi, Yoko Tezuka, Takahiro Kunisada, Toshiyuki Shibata, Ken Ichi Tezuka
Archives of Oral Biology   55 648-654   Jan 2010
The hypoxia condition was expected to be suitable for the establishment and maintenance of human dental pulp cells (hDPCs), because they reside in a low-oxygen environment in vivo. Therefore, we presently examined the effects of hypoxia on the pro...
N. Tamaoki, K. Takahashi, T. Tanaka, T. Ichisaka, H. Aoki, T. Takeda-Kawaguchi, K. Iida, T. Kunisada, T. Shibata, S. Yamanaka, K. Tezuka
J.Dental Res.   89 773-778   2010   [Refereed]
Kazuki Iida, Tomoko Takeda-Kawaguchi, Yoko Tezuka, Takahiro Kunisada, Toshiyuki Shibata, Ken-ichi Tezuka
TEZUKA Kenichi
Arch. Oral Biol.   55 648-654   2010   [Refereed]
T. Takeda, Y. Tezuka, M. Horiuchi, K. Hosono, K. Iida, D. Hatakeyama, S. Miyaki, T. Kunisada, T. Shibata, K. Tezuka
J.Dental Res.   87 676-681   2008   [Refereed]
Ryoji Fujimaki, Ryoji Fujimaki, Katsuhiko Hayashi, Naoko Watanabe, Taketo Yamada, Yoshiaki Toyama, Ken Ichi Tezuka, Nobumichi Hozumi
Journal of Bone and Mineral Metabolism   23 270-273   2005
Spatial and temporal gene inactivation by the cre-loxP system is a powerful tool for analyzing genes of interests. We generated a transgenic mouse line that expresses Cre recombinase under the control of the -742-bp promoter sequence of the mouse ...
Morimura Naoko(Tokyo University of Science),Tezuka Ken-ichi(Tokyo University of Science)
Journal of Biological Chemistry   276(45) 42172-42181   Sep 2001
Cell adhesion molecules are involved in a number of biological functions, such as cell survival, cell differentiation, tissue repair, and development. A novel molecule, POEM (preosteoblast epidermal growth factor-like repeat protein with meprin, A...
Tezuka Ken-ichi(Gifu University Graduate School of Medicine)
Journal of Bone and Mineral Research   17 231-239   Jan 2002
Notch is a transmembrane protein that plays a critical role in the determination of cellular differentiation pathways. Although its importance in the development of mesenchymal tissues has been suggested, its role in skeletal tissues has not been ...
Tezuka Kenichi(Gifu University Graduate School of Medicine)
Key Engineering Materials   243 601-606   Jan 2003
Bone is a highly specialized form of connective tissue consisting of organic and inorganic materials. Bone is continuously remodeled by bone forming osteoblasts and resorbing osteoclasts. In terrestrial vertebrates, these two activities are strict...
Watanabe Naoko(Tokyo University of Science),Tezuka Ken-ichi(Gifu University Graduate School of Medicine)
Journal of Bone and Mineral Metabolism   21 344-352   Mar 2003
Notch is a transmembrane protein involved in cell fate determination. In the present study, we observed temporally and spatially restricted expression of Notch1 in developing cartilage. Notch1 was localized starting from the mesenchymal condensati...
Tezuka Ken-ichi(Gifu University Graduate School of Medicine)
Journal of Bone and Mineral Metabolism   23 1-7   Jan 2005
Bone is a complex system with functions including those of adaptation and repair. To understand how bone cells can create a structure adapted to the mechanical environment, we propose a simple bone remodeling model based on a reaction-diffusion sy...
Sugawara Yasuko(Okayama University),Kamioka Hiroshi(Okayama University),Ken-ichi Tezuka(Gifu University),Takano-Yamamoto Teruko(Okayama University)
Bone   36 877-883   Jan 2005
Osteocytes are surrounded by hard bone matrix. Therefore, it has not previously been possible to demonstrate the real architecture of the osteocyte network in bone. We previously reported that it is possible to observe osteocytes in bone by labeli...
Fujimaki Ryoji(Keio University Dept. Orthopaedic Surgery),Tezuka Ken-ichi(Gifu University Dept. Tissue and Organ Dev.)
Journal of Bone and Mineral Metabolism   24 191-198   Jan 2006
Notch signaling is an evolutionarily conserved mechanism that plays a critical role in the determination of multiple cellular differentiation pathways and morphogenesis during embryogenesis. The limb bud high-density culture is an established mode...
K. Tanaka, T. Matsuo, M. Ohta, T. Sato, K. Tezuka, P. J. Nijweide, Y. Katoh, Y. Hakeda, M. Kumegawa
Mineral and Electrolyte Metabolism   21 189-192   Jan 1995
We succeeded in the isolation of osteocytes from parietal bones of 16- day-old chick embryos. Isolated osteocytes showed a typical stellate morphology. More than 95% of these cells reacted with the osteocyte-specific antibody OB 7.3. In culture os...
G. Wesolowski, L. T. Duong, P. T. Lakkakorpi, R. M. Nagy, K. I. Tezuka, H. Tanaka, G. A. Rodan, S. B. Rodan
Experimental Cell Research   219 679-686   Jan 1995
This study describes the isolation and characterization of highly enriched mammalian osteoclast precursors, released by the 'disintegrin' echistatin, from an osteoclast formation culture. Incubation of a 6-day coculture of mouse bone marrow cells ...
Molecular cloning of a possible Cysteine proteinese predominantly expressed in Osteoclasts.
J. Biol. Chem.   269 1106   1994
H. Kaji, T. Sugimoto, A. Miyauchi, M. Fukase, K. Tezuka, Y. Hakeda, M. Kumegawa, K. Chihara
Endocrinology   135 484-487
Recent evidence indicates that osteopontin (Opn), one of the bone matrix proteins, plays an important role in the attachment of osteoclasts to bone matrix. Besides being elaborated by osteoblasts, this protein is also produced by osteoclasts. The ...
Ken Ichi Tezuka, Ken Ichi Tezuka, Akiyuki Takahashi, Tomoko Takeda, Yoshitaka Wada, Masanori Kikuchi
Key Engineering Materials   306-308 II 1277-1282
Bone is a complex system with adaptation and repair functions. To understand how bone cells can create a structure adapted to the mechanical environment, we proposed a simple bone remodeling model, iBone, based on a reaction-diffusion system [1]. ...
Tomoko Takeda-Kawaguchi, Ken Sugiyama, Shunji Chikusa, Kazuki Iida, Hitomi Aoki, Naritaka Tamaoki, Daijiro Hatakeyama, Takahiro Kunisada, Toshiyuki Shibata, Noemi Fusaki, Ken Ichi Tezuka
PLoS ONE   9 
© 2014 Takeda-Kawaguchi et al. Human dental pulp cells (hDPCs) are a promising resource for regenerative medicine and tissue engineering and can be used for derivation of induced pluripotent stem cells (iPSCs). However, current protocols use reage...
Naoko Yoshimura, Tsutomu Motohashi, Hitomi Aoki, Ken Ichi Tezuka, Natsuki Watanabe, Takanori Wakaoka, Takumi Era, Takahiro Kunisada
Development Growth and Differentiation   55 270-281
Melanocytes are pigment-producing cells generated from neural crest cells (NCCs) that delaminate from the dorsal neural tube. The widely accepted premise that NCCs migrating along the dorsolateral pathway are the main source of melanocytes in the ...
Hiroshi Kamioka, Keishi Ishikawa, Kayo Tanaka, Takuya Sato, Ken Ichi Tezuka, Kenji Hiura, Koji Sumitani, Yoshiyuki Hakeda, Terushige Kawata, Masayoshi Kumegawa
Journal of Bone and Mineral Metabolism   13 3-9
Transforming growth factor-β1 (TGF-β1) has biological functions in various types of cells. However, its roles in the regulation of osteoclast formation and function are unclear. To examine them, we employed a culture system in which unfractionated...
Takuya Sato, Yoshiyuki Hakeda, Yuji Yamaguchi, Hiroshi Mano, Ken Ichi Tezuka, Kunio Matsumoto, Toshikazu Nakamura, Yoshihisa Mori, Kenji Yoshizawa, Koji Sumitani, Hiroaki Kodama, Masayoshi Kumegawa
Journal of Cellular Physiology   164 197-204
Osteoclast formation from hemopoietic precursors has been shown to require the support of stromal cells in bone tissue. In this study, we demonstrated that hepatocyte growth factor (HGF) is one of the stromal cell-derived molecules responsible for...
Ken Ichi Tezuka, Ken Nemoto, Yoko Tezuka, Takuya Sato, Yasushi Ikeda, Masato Kobori, Hiroyuki Kawashima, Hiroshi Eguchi, Yoshiyuki Hakeda, Masayoshi Kumegawa
J. Biol. Chem.   269 15006-15009
Osteoclasts are multinucleate giant cells that play key roles in bone resorption. To identify genes predominantly expressed in osteoclasts, we screened a cDNA library of osteoclasts with cDNA probes of osteoclasts and alveolar macrophages. Clones ...
Ken ichi Tezuka, Takuya Sato, Hiroshi Kamioka, Peter J. Nijweide, Kayo Tanaka, Tetsu Matsuo, Mitsue Ohta, Noriyoshi Kurihara, Yoshiyuki Hakeda, Masayoshi Kumegawa
Biochemical and Biophysical Research Communications   186 911-917
Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines; however, little is known about protein factors produced by osteoclas...
Ken ichi Tezuka, Sunao Takeshita, Yoshiyuki Hakeda, Masayoshi Kumegawa, Reiko Kikuno, Tamotsu Hashimoto-Gotoh
Biochemical and Biophysical Research Communications   173 246-251
Using the differential hybridization screening method between osteoblastic and fibroblastic cells, a cDNA clone coding for an osteoblast specific protein, named OSF-1, consisting of 168 amino acid residues including a possible 32 amino acid long l...
Ken Ichi Tezuka, Yoko Tezuka, Akiko Maejima, Takuya Sato, Ken Nemoto, Hiroshi Kamioka, Yoshiyuki Hakeda, Masayoshi Kumegawa
Journal of Biological Chemistry   269 1106-1109
Osteoclasts are multinucleate giant cells playing key roles in bone resorption. These cells solubilize mineralized bone matrix by means of acid and protease action; however, the precise mechanism of this process is not well known. Recently, we suc...
Tetsuya Inaoka, Graeme Bilbe, Osamu Ishibashi, Ken Ichi Tezuka, Masayoshi Kumegawa, Toshio Kokubo
Biochemical and Biophysical Research Communications   206 89-96
We have previously cloned a rabbit cDNA clone (OC-2) from an osteoclast cDNA library by the differential screening. OC-2 was found to encode a novel cysteine proteinase, tentatively called cathepsin K, which is predominantly expressed in osteoclas...
Ryoji Fujimaki, Ken ichi Tezuka
Clinical calcium.   16 114-119
Notch is a signaling molecule which plays a critical role in the determination of multiple cellular differentiation pathways and morphogenesis in various biological systems, such as neurogenesis, immune system, and hematopoiesis. However, roles of...
Yoshiyuki Hakeda, Shun Ichi Harada, Toshio Matsumoto, Ken Ichi Tezuka, Kiyoshi Higashino, Hiroaki Kodama, Tamotsu Hashimoto-Goto, Etsuro Ogata, Masayoshi Kumegawa
Journal of Biological Chemistry   266 21044-21050
Prostaglandin F2α (PGF2α) stimulates proliferation of clonal osteoblastic MC3T3-E1 cells mainly via the stimulation of phospholipase C. These cells constitutively produced and secreted insulin-like growth factor I (IGF-I). In addition, a neutraliz...
Ken Ichi Tezuka, David T. Denhardt, Gideon A. Rodan, Shun Ichi Harada
J. Biol. Chem.   271(37) 22713-22717
Osteopontin is an arginine-glycine-aspartic acid-containing cell adhesion protein, which is frequently expressed in transformed cells and is thought to play a role in tumorigenesis. v-Src is a transforming viral oncogene product encoded by Rous sa...
Yoshiyuki Hakeda, Kenji Yoshizawa, Marja Hurley, Hiroshi Kawaguchi, Ken Ichi Tezuka, Kayo Tanaka, Takuya Satoh, Masayoshi Kumegawa
Journal of Cellular Physiology   158 444-450
We examined the relationship between signal transduction and the expression of insulin-like growth factor I (IGF-I), IGF-I receptor level, and IGF binding proteins (IGFBPs) in murine clonal osteoblastic MC3T3-E1 cells. 12-O-Tetradecanoylphorbol-13...
Agasa Miyazono, Agasa Miyazono, Atsushi Yamada, Naoko Morimura, Masamichi Takami, Dai Suzuki, Makoto Kobayashi, Ken ichi Tezuka, Matsuo Yamamoto, Ryutaro Kamijo
FEBS Letters   581 5321-5326
POEM, also called nephronectin, is an extracellular matrix protein that is considered to play a critical role as an adhesion molecule in the development and functioning of various tissues, such as kidneys and bones. In the present study, we examin...
Atsushi Noguchi, Naoko Watanabe, Ryoji Fujimaki, Toshio Kitamura, Yoshihide Hayashizaki, Shigeru Miyaki, Kenichi Tezuka, Nobumichi Hozumi
Journal of Bone and Mineral Metabolism   24 153-157
In endochondral ossification, chondrogenesis precedes bone development. Cartilage differentiation is initiated by the formation of chondrogenic cell condensates. Thus, it is essential to investigate genes expressed at the condensation stage for a ...

Books etc

 
培養細胞実験ハンドブック
Aug 2004   
改訂培養細胞実験ハンドブック
Jan 2009   

Conference Activities & Talks

 
Three-dimensional mandible bone architectures simulated by a reaction-diffusion bone remodeling model.
Tezuka Ken-ichi(Gifu University Graduate School of Medicine)
Oct 2004   

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2011 - 2013    Investigator(s): Ken-ichi TEZUKA
For application of iPS cells for clinical use, safer methods without vector integration are being established. Recently, we used episomal plasmid vector system for this purpose, and succeeded to establish multiple lines in collaboration with Kyoto...
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2011 - 2013
Japan Society for the Promotion of Science: Grants-in-Aid for Scientific Research
Project Year: 2014 - 2015
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2004 - 2005    Investigator(s): Ken-ichi TEZUKA
Bone has a structure adapted to the mechanical environment (Wolff's law). However, the complexity of both physical and biological conditions applied to bone have prevented us from investigating the mechanism as to how bone structure can provide en...
Molecmlar mechanisums of Cell-cell Comunication in bone tissnes

Patents

 
5553178 : EFFICIENT METHOD FOR ESTABLISHING INDUCED PLURIPOTENT STEM CELLS
TEZUKA KENICHI,SHIBATA TOSHIYUKI,KUNISADA TAKAHIRO,TAMAOKI NARITAKA,TAKEDA TOMOKO,YAMANAKA SHINYA,TAKAHASHI KAZUTOSHI
2,732,401 : EFFICIENT METHOD FOR ESTABLISHING INDUCED PLURIPOTENT STEM CELLS
TEZUKA KENICHI,SHIBATA TOSHIYUKI,KUNISADA TAKAHIRO,TAMAOKI NARITAKA,TAKEDA TOMOKO,YAMANAKA SHINYA,TAKAHASHI KAZUTOSHI