論文

査読有り 筆頭著者 責任著者 国際誌
2020年5月1日

Applications of bottom-up human artificial chromosomes in cell research and cell engineering.

Experimental cell research
  • Masashi Ikeno
  • ,
  • Yoshinori Hasegawa

390
1
開始ページ
111793
終了ページ
111793
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.yexcr.2019.111793
出版者・発行元
ELSEVIER INC

Chromosome manipulation is a useful technique in biological science. We have constructed human artificial chromosomes (HACs) based on the transfection of centromeric alphoid DNA precursors into cultured human cells. Moreover, HAC-based technology has been developed into a novel gene expression vector tool for introducing large-size genomic DNA. This technique provides natural expression, as well as stable expression without the gene silencing that often occurs with conventional vectors in mammalian cells. Here we review the properties of HACs, and issues regarding the use of HAC technology for basic and applied research.

Web of Science ® 被引用回数 : 6

リンク情報
DOI
https://doi.org/10.1016/j.yexcr.2019.111793
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31874174
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000525406700003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.yexcr.2019.111793
  • ISSN : 0014-4827
  • eISSN : 1090-2422
  • PubMed ID : 31874174
  • Web of Science ID : WOS:000525406700003

エクスポート
BibTeX RIS