2010年10月15日
Ability of IDO to attenuate liver injury in α-galactosylceramide- induced hepatitis model
Journal of Immunology
- 巻
- 185
- 号
- 8
- 開始ページ
- 4554
- 終了ページ
- 4560
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.4049/jimmunol.0904173
IDO converts tryptophan to L-kynurenine, and it is noted as a relevant molecule in promoting tolerance and suppressing adaptive immunity. In this study, we examined the effect of IDO in α-galactosylceramide (α-GalCer)-induced hepatitis. The increase in IDO expression in the liver of wild-type (WT) mice administered α-GalCer was confirmed by real-time PCR, Western blotting, and IDO immunohistochemical analysis. The serum alanine aminotransferase levels in IDO-knockout (KO) mice after α-GalCer injection significantly increased compared with those in WT mice. 1-Methyl-D-tryptophan also exacerbated liver injury in this murine hepatitis model. In α-GalCer-induced hepatitis models, TNF-α is critical in the development of liver injury. The mRNA expression and protein level of TNF-α in the liver from IDO-KO mice were more enhanced compared with those in WT mice. The phenotypes of intrahepatic lymphocytes from WT mice and IDO-KO mice treated with α-GalCer were analyzed by flow cytometry, and the numbers of CD49b+ and CD11b+ cells were found to have increased in IDO-KO mice. Moreover, as a result of the increase in the number of NK cells and macrophages in the liver of IDO-KO mice injected with α-GalCer, TNF-α secretion in these mice was greater than that in WT mice. Deficiency of IDO exacerbated liver injury in α-GalCer-induced hepatitis. IDO induced by proinflammatory cytokines may decrease the number of TNF-α-producing immune cells in the liver. Thus, IDO may suppress overactive immune response in the α-GalCer-induced hepatitis model. Copyright © 2010 by The American Association of Immunologists, Inc.
- リンク情報
-
- DOI
- https://doi.org/10.4049/jimmunol.0904173
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20844202
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78049522618&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=78049522618&origin=inward
- ID情報
-
- DOI : 10.4049/jimmunol.0904173
- ISSN : 0022-1767
- ISSN : 1550-6606
- eISSN : 1550-6606
- PubMed ID : 20844202
- SCOPUS ID : 78049522618