2017年12月
Development of a fluorogenic small substrate for dipeptidyl peptidase-4
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
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- 巻
- 13
- 号
- 開始ページ
- 2690
- 終了ページ
- 2697
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3762/bjoc.13.267
- 出版者・発行元
- BEILSTEIN-INSTITUT
A series of aniline and m-phenylenediamine derivatives with electron-withdrawing 3,3,3-trifluoropropenyl substituents were synthesized as small and chemically stable fluorescent organic compounds. Their fluorescence performances were evaluated by converting 2,4-disubstituted aniline 1 to the non-fluorescent dipeptide analogue H-Gly-Pro-1 for the use as a fluorogenic substrate for dipeptidyl peptidase-4 (DPP-4). The progress of the enzymatic hydrolysis of H-Gly-Pro-1 with DPP-4 was monitored by fluorometric determination of 1 released into the reaction medium. The results suggest that 1 could be used as fluorophore in OFF-ON-type fluorogenic probes.
- リンク情報
- ID情報
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- DOI : 10.3762/bjoc.13.267
- ISSN : 1860-5397
- Web of Science ID : WOS:000417962400002