MISC

2007年11月

Side population in human uterine myometrium displays phenotypic and functional characteristics of myometrial stem cells

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Masanori Ono
  • Tetsuo Maruyama
  • Hirotaka Masuda
  • Takashi Kajitani
  • Takashi Nagashima
  • Toru Arase
  • Mamoru Ito
  • Kuniaki Ohta
  • Hiroshi Uchida
  • Hironori Asada
  • Yasunori Yoshimura
  • Hideyuki Okano
  • Yumi Matsuzaki
  • 全て表示

104
47
開始ページ
18700
終了ページ
18705
記述言語
英語
掲載種別
DOI
10.1073/pnas.0704472104
出版者・発行元
NATL ACAD SCIENCES

Over the course of pregnancy, the human uterus undergoes a 500- to 1,000-fold increase in volume and a 24-fold increase in weight. The uterine smooth muscle layer or myometrium is remodeled, and both cell hypertrophy and hyperplasia are evident. The origin of the new smooth muscle cells, however, is unclear. They may arise from existing smooth muscle cells, or they may be the product of stem cell differentiation. This study describes a subset of myometrial cells isolated from nonpregnant human myometrium that represents the myometrial stem cell population. This was characterized as side population of myometrial cells (myoSP) by a distinct Hoechst dye efflux pattern. In contrast to the main population of myometrial cells (myoMP), myoSP resided in quiescence, underexpressed or lacked myometrial cell markers, and could proliferate and eventually differentiate into mature myometrial cells in vitro only under low oxygen concentration. Although myoMP displayed mature myometrial phenotypes before and after in vitro cultivation, only myoSP, not myoMP, generated functional human myometrial tissues efficiently when transplanted into the uteri of severely immunodeficient mice. Finally, myoSP were multi-potent and made to differentiate into osteocytes and adipocytes in vitro under the appropriate differentiation-inducing conditions. Thus, myoSP exhibited phenotypic and functional characteristics of myometrial stem cells. Study of myoSP will improve the understanding of myometrial physiology and the pathogenesis of myometrium-derived diseases such as leiomyoma. myoSP may also represent a novel source of biological material that could be used in the reconstruction of not only the human uterus but also other organs as well.

リンク情報
DOI
https://doi.org/10.1073/pnas.0704472104
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000251292500064&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.0704472104
  • ISSN : 0027-8424
  • Web of Science ID : WOS:000251292500064

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