論文

査読有り
2012年6月

Forced expression of stabilized c-Fos in dendritic cells reduces cytokine production and immune responses in vivo

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Ryoko Yoshida
  • ,
  • Mayu Suzuki
  • ,
  • Ryota Sakaguchi
  • ,
  • Eiichi Hasegawa
  • ,
  • Akihiro Kimura
  • ,
  • Takashi Shichita
  • ,
  • Takashi Sekiya
  • ,
  • Hiroshi Shiraishi
  • ,
  • Kouji Shimoda
  • ,
  • Akihiko Yoshimura

423
2
開始ページ
247
終了ページ
252
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2012.05.097
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Intracellular cyclic adenosine monophosphate (CAMP) suppresses innate immunity by inhibiting proinflammatory cytokine production by monocytic cells. We have shown that the transcription factor c-Fos is responsible for cAMP-mediated suppression of inflammatory cytokine production, and that c-Fos protein is stabilized by IKK beta-mediated phosphorylation. We found that S308 is one of the major phosphorylation sites, and that the S308D mutation prolongs c-Fos halflife. To investigate the role of stabilized c-Fos protein in dendritic cells (DCs) in vivo, we generated CD11c-promoter-deriven c-FosS308D transgenic mice. As expected, bone marrow-derived DCs (BMDCs) from these Tg mice produced smaller amounts of inflammatory cytokines, including INF-alpha, IL-12, and IL-23. but higher levels of IL-10, in response to LPS, than those from wild-type (Wt) mice. When T cells were co-cultured with BMDCs from Tg mice, production of Th1 and Th17 cytokines was reduced, although T cell proliferation was not affected. Tg mice demonstrated more resistance to experimental autoimmune encephalomyelitis (EAE) than did Wt mice. These data suggest that c-Fos in DCs plays a suppressive role in certain innate and adaptive immune responses. (C) 2012 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2012.05.097
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306443500008&DestApp=WOS_CPL

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