2005年8月
Engineering of phenylacetaldehyde reductase for efficient substrate conversion in concentrated 2-propanol
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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- 巻
- 71
- 号
- 8
- 開始ページ
- 4713
- 終了ページ
- 4720
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1128/AEM.71.8.4713-4720.2005
- 出版者・発行元
- AMER SOC MICROBIOLOGY
Phenylacetaldehyde reductase (PAR) is suitable for the conversion of various aryl ketones and 2-alkanones to corresponding chiral alcohols. 2-Propanol acts as a substrate solvent and hydrogen donor of coupled cofactor regeneration during the conversion of substrates catalyzed by PAR. To improve the conversion efficiency in high concentrations of substrate and 2-propanol, selection of a PAR mutant library and the subsequent rearrangement of mutations were attempted. With only a single selection round and following the manual combination of advantageous mutations, PAR was successfully adapted for the conversion of high concentrations of substrate with concentrated 2-propanol. This method will be widely applicable for the engineering of enzymes potentially valuable for industry.
- リンク情報
- ID情報
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- DOI : 10.1128/AEM.71.8.4713-4720.2005
- ISSN : 0099-2240
- Web of Science ID : WOS:000231165500070