論文

査読有り 最終著者 責任著者 国際誌
2020年5月1日

Involvement of Nlrp9a/b/c in mouse preimplantation development.

Reproduction (Cambridge, England)
  • Satoko Kanzaki
  • ,
  • Shiori Tamura
  • ,
  • Toshiaki Ito
  • ,
  • Mizuki Wakabayashi
  • ,
  • Koji Saito
  • ,
  • Shigeki Kato
  • ,
  • Yasutaka Ohta
  • ,
  • Yoichi Sekita
  • ,
  • Tohru Kimura

160
2
開始ページ
181
終了ページ
191
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1530/REP-19-0516

Nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing proteins (NRLPs) are central components of the inflammasome. Accumulating evidence has shown that a reproductive clade of NRLPs is predominantly expressed in oocyte to cleavage stage embryos, and participates in mammalian preimplantation development as a component of a multiprotein complex known as the subcortical maternal complex (SCMC). Nlrp9s belong to the reproductive class of NLRPs; Nlrp9b is unique in acting as an inflammasome against rotavirus in intestines. Here we generated mice carrying mutations in all three members of the Nlrp9a/b/c gene (Nlrp9 triple mutant (TMut) mice). When crossed with wild-type males, the Nlrp9 TMut females were fertile, but deliveries with fewer pups were increased in the mutants. Consistent with this, blastocyst development was retarded and lethality to the preimplantation embryos increased in the Nlrp9 TMut females in vivo. Under in vitro culture conditions, the fertilized eggs from the Nlrp9 TMut females exhibited developmental arrest at the 2-cell stage, accompanied by asymmetric cell division. By contrast, double mutant (DMut) oocytes (any genetic combination) did not exhibit the 2-cell block in vitro, showing the functional redundancy of Nlrp9a/b/c. Finally, Nlrp9 could bind to components of the SCMC. These results show that Nlrp9 functions as an immune or reproductive NLRP in a cell-type-dependent manner.

リンク情報
DOI
https://doi.org/10.1530/REP-19-0516
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32413845
ID情報
  • DOI : 10.1530/REP-19-0516
  • PubMed ID : 32413845

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