2006年3月
Redirecting differentiation of hematopoietic progenitors by a transcription factor, GATA-2
BLOOD
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- 巻
- 107
- 号
- 5
- 開始ページ
- 1857
- 終了ページ
- 1863
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1182/blood-2005-06-2527
- 出版者・発行元
- AMER SOC HEMATOLOGY
GATA-2 is a zinc finger transcription factor essential for differentiation of immature hematopoietic cells. We analyzed the function of GATA-2 by a combined method of tetracycline-dependent conditional gene expression and in vitro hematopoietic differentiation from mouse embryonic stem (ES) cells using OP9 stroma cells (OP9 system). In the presence of macrophage colony-stimulating factor (M-CSF), the OP9 system induced macrophage differentiation. GATA-2 expression in this system inhibited macrophage differentiation and redirected the fate of hematopoietic differentiation to other hematopoietic lineages. GATA-2 expression commencing at day 5 or day 6 induced megalkaryocytic or erythroid differentiation, respectively. Expression levels of PU.1, a hematopoietic transcription factor that interferes with GATA-2, appeared to play a critical role in differentiation to megakaryocytic or erythroid lineages. Transcription of PU.1 was affected by histone acetylation induced by binding of GATA-2 to the PU.1 promoter region. This study demonstrates that the function of GATA-2 is modified in a context-dependent manner by expression of PU.1, which in turn is regulated by GATA-2.
- リンク情報
- ID情報
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- DOI : 10.1182/blood-2005-06-2527
- ISSN : 0006-4971
- PubMed ID : 16254139
- Web of Science ID : WOS:000235632700024