2020年10月16日
PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins
Journal of Biological Chemistry
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- 巻
- 295
- 号
- 42
- 開始ページ
- 14501
- 終了ページ
- 14509
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1074/jbc.RA120.014643
PGAP6, also known as TMEM8A, is a phospholipase A2 with specificity to glycosylphosphatidylinositol (GPI) and expressed on the surface of various cells. CRIPTO, a GPI-anchored co-receptor for a morphogenic factor Nodal, is a sensitive substrate of PGAP6. PGAP6-mediated shedding of CRIPTO plays a critical role in an early stage of embryogenesis. In contrast, CRYPTIC, a close family member of CRIPTO, is resistant to PGAP6. In this report, chimeras between CRIPTO and CRYPTIC and truncate mutants of PGAP6 were used to demonstrate that the Cripto-1/FRL1/Cryptic domain of CRIPTO is recognized by an N-terminal domain of PGAP6 for processing. We also report that among 56 human GPI-anchored proteins tested, only glypican 3, prostasin, SPACA4, and contactin-1, in addition to CRIPTO, are sensitive to PGAP6, indicating that PGAP6 has a narrow specificity toward various GPI-anchored proteins.
- リンク情報
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- DOI
- https://doi.org/10.1074/jbc.RA120.014643
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/32816994
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85093706267&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85093706267&origin=inward
- ID情報
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- DOI : 10.1074/jbc.RA120.014643
- ISSN : 0021-9258
- eISSN : 1083-351X
- PubMed ID : 32816994
- SCOPUS ID : 85093706267