MISC

2008年1月

A deconvolution procedure for determination of a fluorescence decay waveform applicable to a band-limited measurement system that has a time delay

MEASUREMENT SCIENCE AND TECHNOLOGY
  • Tetsuo Iwata
  • ,
  • Hironobu Shibata
  • ,
  • Tsutomu Araki

19
1
開始ページ
015601
終了ページ
記述言語
英語
掲載種別
DOI
10.1088/0957-0233/19/1/015601
出版者・発行元
IOP PUBLISHING LTD

We propose a deconvolution procedure used for deriving a true fluorescence decay waveform, which is applicable to a band-limited measurement system that has a time delay. In general, the fluorescence decay response has a time delay over the excitation light pulse mainly due to the wavelength dependence of the time delay of the response of a photomultiplier tube (PMT). Furthermore, the frequency band is limited instrumentally or often should be cut off purposely at a relatively low frequency to eliminate high frequency noise. In order to extrapolate such a band-limited response function and to perform a deconvolution procedure in a frequency domain, finally, we introduce a delay time parameter in the conventional Gerchberg-Papoulis (GP) algorithm. We also demonstrate that the Hilbert-transform (HT)-based extrapolation method that we have proposed quite recently is equivalent to the GP algorithm. Simulation and experimental results show that the new method is effective.

リンク情報
DOI
https://doi.org/10.1088/0957-0233/19/1/015601
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253387500037&DestApp=WOS_CPL
ID情報
  • DOI : 10.1088/0957-0233/19/1/015601
  • ISSN : 0957-0233
  • eISSN : 1361-6501
  • Web of Science ID : WOS:000253387500037

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