2008年1月
A deconvolution procedure for determination of a fluorescence decay waveform applicable to a band-limited measurement system that has a time delay
MEASUREMENT SCIENCE AND TECHNOLOGY
- ,
- ,
- 巻
- 19
- 号
- 1
- 開始ページ
- 015601
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1088/0957-0233/19/1/015601
- 出版者・発行元
- IOP PUBLISHING LTD
We propose a deconvolution procedure used for deriving a true fluorescence decay waveform, which is applicable to a band-limited measurement system that has a time delay. In general, the fluorescence decay response has a time delay over the excitation light pulse mainly due to the wavelength dependence of the time delay of the response of a photomultiplier tube (PMT). Furthermore, the frequency band is limited instrumentally or often should be cut off purposely at a relatively low frequency to eliminate high frequency noise. In order to extrapolate such a band-limited response function and to perform a deconvolution procedure in a frequency domain, finally, we introduce a delay time parameter in the conventional Gerchberg-Papoulis (GP) algorithm. We also demonstrate that the Hilbert-transform (HT)-based extrapolation method that we have proposed quite recently is equivalent to the GP algorithm. Simulation and experimental results show that the new method is effective.
- リンク情報
- ID情報
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- DOI : 10.1088/0957-0233/19/1/015601
- ISSN : 0957-0233
- eISSN : 1361-6501
- Web of Science ID : WOS:000253387500037