OTOI Takeshige

Last updated: Nov 6, 2019 at 11:48

Name	OTOI Takeshige
E-mail	otoitokushima-u.ac.jp
Affiliation	The University of Tokushima
Degree	(BLANK), (BLANK)(Yamaguchi University)
Other affiliation	Yamaguchi University
Research funding number	30311814

Research Interests

In Vitro Fertilization and Genome editing(1)

Research Areas

Animal life science / Veterinary medical science /

Academic & Professional Experience

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2010

2012

Professor, Faculty of Agriculture, YAMAGUCHI UNIVERSITY

2006

2010

United Graduate School of Veterinary Science,Yamaguchi University, YAMAGUCHI UNIVERSITY

Education

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1982

Faculty of Agriculture, Yamaguchi University

1984

Graduate School, Division of Agriculture, Yamaguchi University

Published Papers

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The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts.

Tanihara F, Hirata M, Morikawa S, Nguyen NT, LE QA, Hirano T, Fukumi Y, Abe T, Otoi T

The Journal of reproduction and development 65(5) 475-479 Oct 2019 [Refereed]

Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress.

Nguyen TV, Do LTK, Somfai T, Otoi T, Taniguchi M, Kikuchi K

Animal science journal = Nihon chikusan Gakkaiho Oct 2019 [Refereed]

Genome mutation after the introduction of the gene editing by electroporation of Cas9 protein (GEEP) system into bovine putative zygotes.

Namula Z, Wittayarat M, Hirata M, Hirano T, Nguyen NT, Le QA, Fahrudin M, Tanihara F, Otoi T

In vitro cellular & developmental biology. Animal 55(8) 598-603 Sep 2019 [Refereed]

Tracking the fate of endogenous retrovirus segregation in wild and domestic cats.

Ngo MH, Arnal M, Sumi R, Kawasaki J, Miyake A, Grant CK, Otoi T, Fernández de Luco D, Nishigaki K

Journal of virology Sep 2019 [Refereed]

Generation of CD163-edited pig via electroporation of the CRISPR/Cas9 system into porcine in vitro-fertilized zygotes.

Tanihara F, Hirata M, Nguyen NT, Le QA, Wittayarat M, Fahrudin M, Hirano T, Otoi T

Animal biotechnology 1-8 Sep 2019 [Refereed]

The Relationship between Embryonic Development and the Efficiency of Target Mutations in Porcine Endogenous Retroviruses (PERVs) Pol Genes in Porcine Embryos.

Hirata M, Wittayarat M, Hirano T, Nguyen NT, Le QA, Namula Z, Fahrudin M, Tanihara F, Otoi T

Animals : an open access journal from MDPI 9(9) Aug 2019 [Refereed]

Relationship among ovarian follicular status, developmental competence of oocytes, and anti-Müllerian hormone levels: A comparative study in Japanese wild boar crossbred gilts and Large White gilts.

Tanihara F, Hirata M, Iizuka S, Sairiki S, Nii M, Nguyen NT, Le QA, Hirano T, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 90(6) 712-718 Jun 2019 [Refereed]

Hypothermic storage of porcine zygotes in serum supplemented with chlorogenic acid.

Thi Nguyen N, Hirata M, Tanihara F, Hirano T, Le QA, Nii M, Otoi T

Reproduction in domestic animals = Zuchthygiene 54(5) 750-755 May 2019 [Refereed]

Generation of PDX-1 mutant porcine blastocysts by introducing CRISPR/Cas9-system into porcine zygotes via electroporation.

Tanihara F, Hirata M, Nguyen NT, Le QA, Hirano T, Takemoto T, Nakai M, Fuchimoto DI, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 90(1) 55-61 Oct 2018 [Refereed]

Genome mutation after introduction of the gene editing by electroporation of Cas9 protein (GEEP) system in matured oocytes and putative zygotes.

Hirata M, Tanihara F, Wittayarat M, Hirano T, Nguyen NT, Le QA, Namula Z, Nii M, Otoi T

In vitro cellular & developmental biology. Animal 55(4) 237-242 Apr 2019 [Refereed]

Effects of Tris (hydroxymethyl) aminomethane on the quality of frozen-thawed boar spermatozoa.

Namula Z, Tanihara F, Wittayarat M, Hirata M, Nguyen NT, Hirano T, Le QA, Nii M, Otoi T

Acta veterinaria Hungarica 67(1) 106-114 Mar 2019 [Refereed]

Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos.

Tanihara F, Hirata M, Nguyen NT, LE QA, Hirano T, Otoi T

The Journal of reproduction and development Feb 2019 [Refereed]

Effects of chlorogenic acid and caffeic acid on the quality of frozen-thawed boar sperm.

Namula Z, Hirata M, Wittayarat M, Tanihara F, Thi Nguyen N, Hirano T, Nii M, Otoi T

Reproduction in domestic animals = Zuchthygiene 53(6) 1600-1604 Dec 2018 [Refereed]

Follicular development of canine ovaries stimulated by a combination treatment of eCG and hCG.

Hirata M, Tanihara F, Taniguchi M, Takagi M, Terazono T, Otoi T

Veterinary medicine and science 4(4) 333-340 Nov 2018 [Refereed]

Generation of a TP53-modified porcine cancer model by CRISPR/Cas9-mediated gene modification in porcine zygotes via electroporation.

Tanihara F, Hirata M, Nguyen NT, Le QA, Hirano T, Takemoto T, Nakai M, Fuchimoto DI, Otoi T

PloS one 13(10) e0206360 2018 [Refereed]

Effects of chlorogenic acid (CGA) supplementation during in vitro maturation culture on the development and quality of porcine embryos with electroporation treatment after in vitro fertilization.

Nguyen TV, Wittayarat M, Do LTK, Nguyen TV, Nii M, Namula Z, Kunihara T, Tanihara F, Hirata M, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 89(8) 1207-1213 Aug 2018 [Refereed]

Effect of ferulic acid supplementation on the developmental competence of porcine embryos during in vitro maturation.

Tanihara F, Hirata M, Nhien NT, Hirano T, Kunihara T, Otoi T

The Journal of veterinary medical science 80(6) 1007-1011 Jun 2018 [Refereed]

Effects of voltage strength during electroporation on the development and quality of in vitro-produced porcine embryos.

Nishio K, Tanihara F, Nguyen TV, Kunihara T, Nii M, Hirata M, Takemoto T, Otoi T

Reproduction in domestic animals = Zuchthygiene 53(2) 313-318 Apr 2018 [Refereed]

Effects of Antifreeze Protein Supplementation on the Development of Porcine Morulae Stored at Hypothermic Temperatures.

Nguyen TV, Tanihara F, Hirata M, Hirano T, Nishio K, Kim Do LT, Nguyen TV, Nii M, Otoi T

Cryo letters 39(2) 131-136 Mar 2018 [Refereed]

Comparative Effects of Different Dosages of hCG on Follicular Development in Postpartum Dairy Cows With Cystic Ovarian Follicles.

Ono T, Takagi M, Kawashima C, Wijayagunawardane MPB, Vos PLAM, Taniguchi M, Tanihara F, Otoi T

Frontiers in veterinary science 5 130 2018 [Refereed]

Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes.

Nguyen TV, Tanihara F, Do L, Sato Y, Taniguchi M, Takagi M, Van Nguyen T, Otoi T

Reproduction in domestic animals = Zuchthygiene 52(6) 969-975 Dec 2017 [Refereed]

Epigenetic modulation on cat-cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A.

Wittayarat M, Sato Y, Do LT, Chatdarong K, Tharasanit T, Techakumphu M, Taniguchi M, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 88(4) 593-601 Apr 2017 [Refereed]

Sensitivity of the meiotic stage to hyperthermia during in vitro maturation of porcine oocytes.

Nishio K, Yamazaki M, Taniguchi M, Besshi K, Morita F, Kunihara T, Tanihara F, Takemoto T, Otoi T

Acta veterinaria Hungarica 65(1) 115-123 Mar 2017 [Refereed]

Effects of duration of electric pulse on in vitro development of cloned cat embryos with human artificial chromosome vector.

Do L, Wittayarat M, Terazono T, Sato Y, Taniguchi M, Tanihara F, Takemoto T, Kazuki Y, Kazuki K, Oshimura M, Otoi T

Reproduction in domestic animals = Zuchthygiene 51(6) 1039-1043 Dec 2016 [Refereed]

Existence of Two Distinct Infectious Endogenous Retroviruses in Domestic Cats and Their Different Strategies for Adaptation to Transcriptional Regulation.

Kuse K, Ito J, Miyake A, Kawasaki J, Watanabe S, Makundi I, Ngo MH, Otoi T, Nishigaki K

Journal of virology 90(20) 9029-9045 Oct 2016 [Refereed]

Somatic cell reprogramming-free generation of genetically modified pigs.

Tanihara F, Takemoto T, Kitagawa E, Rao S, Do LT, Onishi A, Yamashita Y, Kosugi C, Suzuki H, Sembon S, Suzuki S, Nakai M, Hashimoto M, Yasue A, Matsuhisa M, Noji S, Fujimura T, Fuchimoto D, Otoi T

Science advances 2(9) e1600803 Sep 2016 [Refereed]

The optimal period of Ca-EDTA treatment for parthenogenetic activation of porcine oocytes during maturation culture.

Morita Y, Taniguchi M, Tanihara F, Ito A, Namula Z, DO LT, Takagi M, Takemoto T, Otoi T

The Journal of veterinary medical science 78(6) 1019-1023 Jul 2016 [Refereed]

CHARACTERISTICS AND FERTILITY OF SUMATRAN TIGER SPERMATOZOA CRYOPRESERVED WITH DIFFERENT SUGARS.

Wayan Kurniani Karja N, Fahrudin M, Setiadi MA, Tumbelaka LI, Sudarwati R, Hastuti YT, Mulia BH, Widianti A, Sultan K, Terazono T, Namula Z, Taniguchi M, Tanihara F, Takemoto T, Kikuchi K, Sato Y, Otoi T

Cryo letters 37(4) 264-271 Jul 2016 [Refereed]

Melatonin Supplementation During In Vitro Maturation and Development Supports the Development of Porcine Embryos.

Do LT, Shibata Y, Taniguchi M, Nii M, Nguyen TV, Tanihara F, Takagi M, Otoi T

Reproduction in domestic animals = Zuchthygiene 50(6) 1054-1058 Dec 2015 [Refereed]

Effects of dibutyryl cyclic adenosine monophosphate and human chorionic gonadotropin on the formation of antral follicle-like structures by bovine cumulus-oocyte complexes.

Ono T, Takaoka A, Morita Y, Do L, Tanihara F, Taniguchi M, Otoi T

Acta veterinaria Hungarica 63(4) 485-498 Dec 2015 [Refereed]

Effects of Dietary Contamination by Zearalenone and Its Metabolites on Serum Anti-Müllerian Hormone: Impact on the Reproductive Performance of Breeding Cows.

Fushimi Y, Takagi M, Monniaux D, Uno S, Kokushi E, Shinya U, Kawashima C, Otoi T, Deguchi E, Fink-Gremmels J

Reproduction in domestic animals = Zuchthygiene 50(5) 834-839 Oct 2015 [Refereed]

In vitro development of OPU-derived bovine embryos cultured either individually or in groups with the silk protein sericin and the viability of frozen-thawed embryos after transfer.

Isobe T, Ikebata Y, Do LT, Tanihara F, Taniguchi M, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 86(7) 661-665 Jul 2015 [Refereed]

EFFECTS OF ORVUS ES PASTE ON THE MOTILITY AND VIABILITY OF YAK (BOS GRUNNIENS) EPIDIDYMAL AND EJACULATED SPERMATOZOA AFTER FREEZING AND THAWING.

Shimazaki M, Sambuu R, Sato Y, Kim Do LT, Tanihara F, Taniguchi M, Otoi T

Cryo letters 36(4) 264-269 Jul 2015 [Refereed]

Astaxanthin present in the maturation medium reduces negative effects of heat shock on the developmental competence of porcine oocytes.

Do LT, Luu VV, Morita Y, Taniguchi M, Nii M, Peter AT, Otoi T

Reproductive biology 15(2) 86-93 Jun 2015 [Refereed]

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin.

Yasmin C, Otoi T, Setiadi MA, Karja NW

Acta veterinaria Hungarica 63(1) 110-117 Mar 2015 [Refereed]

Formation of an Antral Follicle-Like Structure by Bovine Cumulus-Oocyte Complexes Embedded with Fragmin/Protamine Microparticles.

Morita Y, Karja NW, Do LT, Tanihara F, Taniguchi M, Otoi T

Animal biotechnology 26(4) 273-275 2015 [Refereed]

Chelation of trace elements in preservation medium influences the quality of boar spermatozoa during liquid preservation at 5°C for 4 weeks.

Taniguchi M, Wittayarat M, Morinaga K, Sato Y, Kin Do LT, Chatdarong K, Techakumphu M, Nii M, Otoi T

Cryo letters 35(4) 336-344 Jul 2014 [Refereed]

Cell cycle analysis and interspecies nuclear transfer of cat cells treated with chemical inhibitors.

Wittayarat M, Fujiwara A, Chatdarong K, Techakumphu M, Sato Y, Tanihara F, Morita Y, Taniguchi M, Otoi T

Acta veterinaria Hungarica 62(2) 233-242 Jun 2014 [Refereed]

Roles of the zona pellucida and functional exposure of the sperm-egg fusion factor 'IZUMO' during in vitro fertilization in pigs.

Tanihara F, Nakai M, Men NT, Kato N, Kaneko H, Noguchi J, Otoi T, Kikuchi K

Animal science journal = Nihon chikusan Gakkaiho 85(4) 395-404 Apr 2014 [Refereed]

Effect of sericin supplementation during in vitro maturation on the maturation, fertilization and development of porcine oocytes.

Do LT, Namula Z, Luu VV, Sato Y, Taniguchi M, Isobe T, Kikuchi K, Otoi T

Reproduction in domestic animals = Zuchthygiene 49(2) e17-20 Apr 2014 [Refereed]

Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C.

Namula Z, Kodama R, Tanihara F, Morita Y, Sato Y, Wittayarat M, Taniguchi M, Otoi T

Acta veterinaria Hungarica 62(1) 106-116 Mar 2014 [Refereed]

Nuclear status and DNA fragmentation of oocytes from porcine, bovine and feline ovaries stored at 4 degrees C for 5 days.

Luu VV, Namula Z, Do LT, Sato Y, Taniguchi M, Karja NW, Otoi T

Cryo letters 35(1) 48-53 Jan 2014 [Refereed]

Retrospective surveillance of metabolic parameters affecting reproductive performance of Japanese Black breeding cows.

Watanabe U, Takagi M, Yamato O, Otoi T, Okamoto K

Journal of veterinary science 15(2) 283-288 2014 [Refereed]

Comparison of activation ability between feline and bovine oocytes.

Tanihara F, Kaedei Y, Namula Z, Luu VV, Sato Y, Wittayarat M, Taniguchi M, Otoi T

Acta veterinaria Hungarica 61(4) 491-494 Dec 2013 [Refereed]

Cryopreservation for bovine embryos in serum-free freezing medium containing silk protein sericin.

Isobe T, Ikebata Y, Onitsuka T, Do LT, Sato Y, Taniguchi M, Otoi T

Cryobiology 67(2) 184-187 Oct 2013 [Refereed]

Effects of green tea polyphenol on the quality of canine semen after long-term storage at 5°C.

Wittayarat M, Ito A, Kimura T, Namula Z, Luu VV, Do LT, Sato Y, Taniguchi M, Otoi T

Reproductive biology 13(3) 251-254 Sep 2013 [Refereed]

Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks.

Namula Z, Sato Y, Kodama R, Morinaga K, Luu VV, Taniguchi M, Nakai M, Tanihara F, Kikuchi K, Nagai T, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 84(8) 600-606 Aug 2013 [Refereed]

Histone deacetylase inhibitor improves the development and acetylation levels of cat-cow interspecies cloned embryos.

Wittayarat M, Sato Y, Do LT, Morita Y, Chatdarong K, Techakumphu M, Taniguchi M, Otoi T

Cellular reprogramming 15(4) 301-308 Aug 2013 [Refereed]

Novel iontophoretic administration method for local therapy of breast cancer.

Komuro M, Suzuki K, Kanebako M, Kawahara T, Otoi T, Kitazato K, Inagi T, Makino K, Toi M, Terada H

Journal of controlled release : official journal of the Controlled Release Society 168(3) 298-306 Jun 2013 [Refereed]

The effect of relaxin supplementation of in vitro maturation medium on the development of cat oocytes obtained from ovaries stored at 4°C.

Luu VV, Hanatate K, Tanihara F, Sato Y, Do LT, Taniguchi M, Otoi T

Reproductive biology 13(2) 122-126 Jun 2013 [Refereed]

Metabolic profile of Japanese Black breeding cattle herds: usefulness in selection for nutrient supplementation to enhance reproductive performance and regional differences.

Watanabe U, Takagi M, Yamato O, Otoi T, Tshering C, Okamoto K

The Journal of veterinary medical science 75(4) 481-487 May 2013 [Refereed]

Establishment of adult mouse Sertoli cell lines by using the starvation method.

Sato Y, Yoshida K, Nozawa S, Yoshiike M, Arai M, Otoi T, Iwamoto T

Reproduction (Cambridge, England) 145(5) 505-516 May 2013 [Refereed]

Japanese Black breeding herd exhibiting low blood urea nitrogen: a metabolic profile study examining the effect on reproductive performance.

Watanabe U, Okamoto K, Miyamoto A, Otoi T, Yamato O, Tshering C, Takagi M

Animal science journal = Nihon chikusan Gakkaiho 84(5) 389-394 May 2013 [Refereed]

Cell cycle synchronization of skin fibroblast cells in four species of family Felidae.

Wittayarat M, Thongphakdee A, Saikhun K, Chatdarong K, Otoi T, Techakumphu M

Reproduction in domestic animals = Zuchthygiene 48(2) 305-310 Apr 2013 [Refereed]

Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium.

Sambuu R, Takagi M, Namula Z, Nii M, Taniguchi M, Uno S, Kokushi E, Tshering C, dos Santos RR, Fink-Gremmels J, Otoi T

Animal science journal = Nihon chikusan Gakkaiho 84(1) 28-34 Jan 2013 [Refereed]

In vitro fertilization and development of porcine oocytes matured in follicular fluid.

Agung B, Otoi T, Fuchimoto D, Senbon S, Onishi A, Nagai T

The Journal of reproduction and development 59(2) 103-106 2013 [Refereed]

Evaluation of zona pellucida function for sperm penetration during in vitro fertilization in pigs.

Tanihara F, Nakai M, Kaneko H, Noguchi J, Otoi T, Kikuchi K

The Journal of reproduction and development 59(4) 385-392 2013 [Refereed]

Effects of (-)-epigallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium.

Kaedei Y, Naito M, Naoi H, Sato Y, Taniguchi M, Tanihara F, Kikuchi K, Nagai T, Otoi T

Reproduction in domestic animals = Zuchthygiene 47(6) 880-886 Dec 2012 [Refereed]

Effect of sericin on preimplantation development of bovine embryos cultured individually.

Isobe T, Ikebata Y, Onitsuka T, Wittayarat M, Sato Y, Taniguchi M, Otoi T

Theriogenology 78(4) 747-752 Sep 2012 [Refereed]

Long-term preservation of chilled canine semen using vitamin C in combination with green tea polyphenol.

Wittayarat M, Kimura T, Kodama R, Namula Z, Chatdarong K, Techakumphu M, Sato Y, Taniguchi M, Otoi T

Cryo letters 33(4) 318-326 Jul 2012 [Refereed]

Follicle formation in the canine ovary after autografting to a peripheral site.

Terazono T, Kaedei Y, Tanihara F, Namula Z, Viet V, Takagi M, Inoue M, Sato Y, Taniguchi M, Otoi T

Reproduction in domestic animals = Zuchthygiene 47(2) e16-21 Apr 2012 [Refereed]

Glycoconjugates recognized by peanut agglutinin lectin in the inner acellular layer of the lamina propria of seminiferous tubules in human testes showing impaired spermatogenesis.

Sato Y, Nozawa S, Yoshiike M, Otoi T, Iwamoto T

Human reproduction (Oxford, England) 27(3) 659-668 Mar 2012 [Refereed]

Assessment of canine ovaries autografted to various body sites.

Terazono T, Inoue M, Kaedei Y, Tanihara F, Namula Z, Viet VL, Taura Y, Takagi M, Takuma T, Otoi T

Theriogenology 77(1) 131-138 Jan 2012 [Refereed]

Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media.

Murakami M, Dong YJ, Suzuki T, Taniguchi M, Kaedei Y, Sato Y, Tanihara F, Otoi T

Cryobiology 63(3) 170-174 Dec 2011 [Refereed]

Effects of exposure to zearalenone on porcine oocytes and sperm during maturation and fertilization in vitro.

Sambuu R, Takagi M, Namula Z, Otoi T, Shiga S, Rodrigues Dos Santos R, Fink-Gremmels J

The Journal of reproduction and development 57(4) 547-550 Sep 2011 [Refereed]

Assisted hatching of poor-quality bovine embryos increases pregnancy success rate after embryo transfer.

Taniyama A, Watanabe Y, Nishino Y, Inoue T, Taura Y, Takagi M, Kubota C, Otoi T

The Journal of reproduction and development 57(4) 543-546 Sep 2011 [Refereed]

Antiviral restriction factor transgenesis in the domestic cat.

Wongsrikeao P, Saenz D, Rinkoski T, Otoi T, Poeschla E

Nature methods 8(10) 853-859 Sep 2011 [Refereed]

Effects of cryoprotectant agents and equilibration methods on developmental competence of porcine oocytes.

Taniguchi M, Arikawa R, Kaedei Y, Tanihara F, Namula Z, Viet VL, Sato Y, Otoi T

Cryo letters 32(5) 410-414 Sep 2011 [Refereed]

Formation of an antral follicle-like structure of bovine cumulus-oocyte complexes embedded individually or in groups in collagen gels.

Abakushina EV, Morita Y, Kaedei Y, Tanihara F, Namula Z, Viet VL, Otoi T

Reproduction in domestic animals = Zuchthygiene 46(3) 423-427 Jun 2011 [Refereed]

Detection of zearalenone and its metabolites in naturally contaminated porcine follicular fluid by using liquid chromatography-tandem mass spectrometry.

Sambuu R, Takagi M, Shiga S, Uno S, Kokushi E, Namula Z, Otoi T, Miyamoto A, Deguchi E, Fink-Gremmels J

The Journal of reproduction and development 57(2) 303-306 Apr 2011 [Refereed]

Combination of the somatic cell nuclear transfer method and RNAi technology for the production of a prion gene-knockdown calf using plasmid vectors harboring the U6 or tRNA promoter.

Wongsrikeao P, Sutou S, Kunishi M, Dong YJ, Bai X, Otoi T

Prion 5(1) 39-46 Jan 2011 [Refereed]

In vitro maturation and development of porcine oocytes cultured in a straw or dish using a portable incubator with a CO2 chamber.

Fujii A, Kaedei Y, Tanihara F, Ito A, Hanatate K, Kikuchi K, Nagai T, Otoi T

Reproduction in domestic animals = Zuchthygiene 45(4) 619-624 Aug 2010 [Refereed]

Effects of oxygen tension and follicle cells on maturation and fertilization of porcine oocytes during in vitro culture in follicular fluid.

Agung B, Piao Y, Fuchimoto D, Senbon S, Onishi A, Otoi T, Nagai T

Theriogenology 73(7) 893-899 Apr 2010 [Refereed]

Plasma progesterone profiles in Beagle bitches with and without the whelping experience.

Seki M, Watanabe N, Ishii K, Kinoshita Y, Aihara T, Takeiri S, Otoi T

Acta veterinaria Hungarica 58(1) 117-124 Mar 2010 [Refereed]

Effects of twice-weekly follicular punctures of ovaries with or without the corpus luteum on follicular and luteal dynamics.

Takuma T, Otsubo T, Kurokawa Y, Ichimaru H, Otoi T

Reproduction in domestic animals = Zuchthygiene 45(1) 50-54 Feb 2010 [Refereed]

Effects of season and reproductive phase on the quality, quantity and developmental competence of oocytes aspirated from Japanese black cows.

Takuma T, Sakai S, Ezoe D, Ichimaru H, Jinnouchi T, Kaedei Y, Nagai T, Otoi T

The Journal of reproduction and development 56(1) 55-59 Feb 2010 [Refereed]

Influence of parity and litter size on gestation length in beagle dogs.

Seki M, Watanabe N, Ishii K, Kinoshita Y, Aihara T, Takeiri S, Otoi T

Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 74(1) 78-80 Jan 2010 [Refereed]

Influence of freezing with liquid nitrogen on whole-knee joint grafts and protection of cartilage from cryoinjury in rabbits.

Hayashi M, Tsuchiya H, Otoi T, Agung B, Yamamoto N, Tomita K

Cryobiology 59(1) 28-35 Aug 2009 [Refereed]

Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle.

Takagi M, Mukai S, Kuriyagawa T, Takagaki K, Uno S, Kokushi E, Otoi T, Budiyanto A, Shirasuna K, Miyamoto A, Kawamura O, Okamoto K, Deguchi E

Reproductive toxicology (Elmsford, N.Y.) 26(2) 164-169 Oct 2008 [Refereed]

Messenger RNA expression of angiotensin-converting enzyme, endothelin, cyclooxygenase-2 and prostaglandin synthases in bovine placentomes during gestation and the postpartum period.

Takagi M, Yamamoto D, Ogawa S, Otoi T, Ohtani M, Miyamoto A

Veterinary journal (London, England : 1997) 177(3) 398-404 Sep 2008 [Refereed]

Meiotic competence and DNA damage of porcine oocytes exposed to an elevated temperature.

Barati F, Agung B, Wongsrikeao P, Taniguchi M, Nagai T, Otoi T

Theriogenology 69(6) 767-772 Apr 2008 [Refereed]

Effects of the reproductive status on morphological oocyte quality and developmental competence of oocytes after in vitro fertilization and somatic cell nuclear transfer in cat.

Naoi H, Agung B, Karja NW, Wongsrikeao P, Shimizu R, Taniguchi M, Otoi T

Reproduction in domestic animals = Zuchthygiene 43(2) 157-161 Apr 2008 [Refereed]

Establishment of a novel equine cell line for isolation and propagation of equine herpesviruses.

Maeda K, Yasumoto S, Tsuruda A, Andoh K, Kai K, Otoi T, Matsumura T

The Journal of veterinary medical science 69(9) 989-991 Sep 2007 [Refereed]

Effect of cryoprotectant composition on in vitro viability of in vitro fertilized and cloned bovine embryos following vitrification and in-straw dilution.

Taniguchi M, Ikeda A, Arikawa E, Wongsrikeao P, Agung B, Naoi H, Nagai T, Otoi T

The Journal of reproduction and development 53(4) 963-969 Aug 2007 [Refereed]

Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology.

Sutou S, Kunishi M, Kudo T, Wongsrikeao P, Miyagishi M, Otoi T

BMC biotechnology 7 44 Jul 2007 [Refereed]

Ovarian follicular and corpus luteum changes, progesterone concentrations, estrus and ovulation following estradiol benzoate/progesterone based treatment protocol in cross-bred cows.

Taniguchi M, Ikeda A, Arikawa E, Shimizu R, Seki M, Karaki M, Rajamahendran R, Otoi T

Animal reproduction science 99(3-4) 389-394 Jun 2007 [Refereed]

Improvement of transgenic cloning efficiencies by culturing recipient oocytes and donor cells with antioxidant vitamins in cattle.

Wongsrikeao P, Nagai T, Agung B, Taniguchi M, Kunishi M, Suto S, Otoi T

Molecular reproduction and development 74(6) 694-702 Jun 2007 [Refereed]

Developmental competence of cat oocytes from ovaries stored at various temperature for 24 h.

Naoi H, Otoi T, Shimamura T, Karja NW, Agung B, Shimizu R, Taniguchi M, Nagai T

The Journal of reproduction and development 53(2) 271-277 Apr 2007 [Refereed]

Role of cumulus cells on in vitro maturation of canine oocytes.

Otoi T, Shin T, Kraemer DC, Westhusin ME

Reproduction in domestic animals = Zuchthygiene 42(2) 184-189 Apr 2007 [Refereed]

Effects of electric field strengths on fusion and in vitro development of domestic cat embryos derived by somatic cell nuclear transfer.

Karja NW, Otoi T, Wongsrikeao P, Shimizu R, Murakami M, Agung B, Fahrudin M, Nagai T

Theriogenology 66(5) 1237-1242 Sep 2006 [Refereed]

Effects of single and double exposure to brilliant cresyl blue on the selection of porcine oocytes for in vitro production of embryos.

Wongsrikeao P, Otoi T, Yamasaki H, Agung B, Taniguchi M, Naoi H, Shimizu R, Nagai T

Theriogenology 66(2) 366-372 Jul 2006 [Refereed]

Effects of beta-mercaptoethanol and cycloheximide on survival and DNA damage of bovine embryos stored at 4 degrees C for 72 h.

Mori M, Otoi T, Wongsrikeao P, Agung B, Nagai T

Theriogenology 65(7) 1322-1332 Apr 2006 [Refereed]

Effect of maturation culture period of oocytes on the sex ratio of in vitro fertilized bovine embryos.

Agung B, Otoi T, Wongsrikeao P, Taniguchi M, Shimizu R, Watari H, Nagai T

The Journal of reproduction and development 52(1) 123-127 Feb 2006 [Refereed]

Meiotic competence of canine oocytes embedded in collagen gel.

Otoi T, Shimizu R, Naoi H, Wongsrikeao P, Agung B, Taniguchi M

Reproduction in domestic animals = Zuchthygiene 41(1) 17-21 Feb 2006 [Refereed]

Effects of hexoses on in vitro oocyte maturation and embryo development in pigs.

Wongsrikeao P, Otoi T, Taniguchi M, Karja NW, Agung B, Nii M, Nagai T

Theriogenology 65(2) 332-343 Jan 2006 [Refereed]

In vitro development and post-thaw survival of blastocysts derived from delipidated zygotes from domestic cats.

Karja NW, Otoi T, Wongsrikeao P, Murakami M, Agung B, Fahrudin M, Nagai T

Theriogenology 65(2) 415-423 Jan 2006 [Refereed]

Effect of cycloheximide on in vitro development of electrically activated feline oocytes.

Karja NW, Otoi T, Murakami M, Wongsrikeao P, Budiyanto A, Fahrudin M, Nagai T

The Journal of reproduction and development 51(6) 783-786 Dec 2005 [Refereed]

Development of cat embryos produced by intracytoplasmic injection of spermatozoa stored in alcohol.

Murakami M, Karja NW, Wongsrikeao P, Agung B, Taniguchi M, Naoi H, Otoi T

Reproduction in domestic animals = Zuchthygiene 40(6) 511-515 Dec 2005 [Refereed]

Analysis of DNA fragmentation of porcine embryos exposed to cryoprotectants.

Rajaei F, Karja NW, Agung B, Wongsrikeao P, Taniguchi M, Murakami M, Sambuu R, Nii M, Otoi T

Reproduction in domestic animals = Zuchthygiene 40(5) 429-432 Oct 2005 [Refereed]

Effect of the removal of cumulus cells on the nuclear maturation, fertilization and development of porcine oocytes.

Wongsrikeao P, Kaneshige Y, Ooki R, Taniguchi M, Agung B, Nii M, Otoi T

Reproduction in domestic animals = Zuchthygiene 40(2) 166-170 Apr 2005 [Refereed]

Teaching Experience

Food and Life (YAMAGUCHI UNIVERSITY)
Introductory Seminar 1 (YAMAGUCHI UNIVERSITY)

Association Memberships

International Society for Transgenic Technologies(3) , THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE(429) , Japan Embryo Transfer Society(24) , JAPAN SOCIETY FOR OVA RESEARCH(42) , SOCIETY FOR REPRODUCTION AND DEVELOPMENT(102) , THE JAPANESE SOCIETY OF VETERINARY SCIENCE(382)