2017年5月
Profile differences in tetrodotoxin transfer to skin and liver in the pufferfish Takifugu rubripes
TOXICON
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- 巻
- 130
- 号
- 開始ページ
- 73
- 終了ページ
- 78
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.toxicon.2017.03.001
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
Tetrodotoxin (TTX) was intramuscularly administered to nontoxic cultured specimens of the pufferfish Takifugu rubripes to investigate differences in the toxin transfer and accumulation profiles between the skin and liver. Test fish were administered TTX at doses of 30 (Low dose: LD), 100 (Medium dose: MD), and 300 (High dose; HD) mu g/individual, respectively. Liquid chromatography/mass spectrometry analysis for TTX revealed that the TTX concentration in both the skin (0.48-1.7 mu g/g) and liver (0.43-6.0 mu g/g) at 24 h after the toxin administration increased with an increase in the dose. The ID( accumulation ratio (ratio (%) of accumulated TTX in each tissue (mu g/tissue) to the administered dose (mu g/individual)) of the skin (11.1-38.6) significantly decreased with an increase in the dose, whereas that of the liver (18.4 - 21.3) was almost constant irrespective of the dose. Immunohistochemical observations of the skin sections revealed TTX-positive signals in basal cells in LD, but as the dose increased, TTX-positive signals were also observed in the epidermis. In the liver sections, TTX-positive signals were rarely observed in LD and MD, but the TTX was distributed throughout the liver tissue in HD. (C) 2017 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.toxicon.2017.03.001
- ISSN : 0041-0101
- PubMed ID : 28263757
- Web of Science ID : WOS:000399510700010