論文

査読有り
2019年12月5日

Tetrodotoxin functions as a stress relieving substance in juvenile tiger puffer Takifugu rubripes

Toxicon
  • Masafumi Amano
  • ,
  • Noriko Amiya
  • ,
  • Minami Takaoka
  • ,
  • Haruka Sato
  • ,
  • Tomohiro Takatani
  • ,
  • Osamu Arakawa
  • ,
  • Yoshitaka Sakakura

171
開始ページ
54
終了ページ
61
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.toxicon.2019.09.024

© 2019 Elsevier Ltd We tested whether tetrodotoxin (TTX) functions as a stress relieving substance in puffer fish. We orally administered TTX to the juveniles of hatchery-reared non-toxic tiger puffer Takifugu rubripes and measured the effects of TTX on brain corticotropin-releasing hormone (CRH) mRNA expression and plasma cortisol levels in comparison with effects in non-toxic juveniles. Firstly, the reciprocal connections of CRH and adrenocorticotropic hormone (ACTH) were confirmed by dual-label immunohistochemistry. CRH-immunoreactive (ir) cell bodies were detected in the hypothalamus and CRH-ir fibers were observed to project to ACTH-ir cells in the rostral pars distalis of the pituitary. Next, a TTX-containing diet (2.35 mouse units (517 ng)/g diet) or a non-toxic diet were fed to the fish for 28 days under a recirculating system. Standard length and body weight became significantly larger in the TTX-treated group. The degree of loss of the caudal fin, which is an indicator of the degree of agonistic interactions, where high values show a higher loss of caudal fin of a fish due to nipping by other individuals, was significantly lower in the TTX-treated group. Relative CRH mRNA expression levels in the brain and cortisol levels in the plasma were significantly lower in the TTX-treated group. These results indicate that TTX functions as a stress relieving substance by affecting the CRH-ACTH-cortisol axis and reducing agonistic interactions in tiger puffer juveniles.

リンク情報
DOI
https://doi.org/10.1016/j.toxicon.2019.09.024
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31580836
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-85073215262&origin=inward
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85073215262&origin=inward
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