論文

査読有り
2020年6月

The planocerid flatworm is a main supplier of toxin to tetrodotoxin-bearing fish juveniles

Chemosphere
  • Shiro Itoi
  • ,
  • Tatsunori Sato
  • ,
  • Mitsuki Takei
  • ,
  • Riko Yamada
  • ,
  • Ryuya Ogata
  • ,
  • Hikaru Oyama
  • ,
  • Shun Teranishi
  • ,
  • Ayano Kishiki
  • ,
  • Takenori Wada
  • ,
  • Kaede Noguchi
  • ,
  • Misato Abe
  • ,
  • Taiki Okabe
  • ,
  • Hiroyuki Akagi
  • ,
  • Maho Kashitani
  • ,
  • Rei Suo
  • ,
  • Tomoko Koito
  • ,
  • Tomohiro Takatani
  • ,
  • Osamu Arakawa
  • ,
  • Haruo Sugita

249
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.chemosphere.2020.126217

© 2020 Elsevier Ltd Tetrodotoxin (TTX), a potent neurotoxin, is found in various phylogenetically diverse taxa. In marine environments, the pufferfish is at the top of the food chain among TTX-bearing organisms. The accumulation of TTX in the body of pufferfish appears to be of the food web that begins with bacteria. It is known that toxic pufferfishes possess TTX from the larval/juvenile stage. However, the source of the TTX is unknown because the maternally sourced TTX is extremely small in quantity. Therefore, the TTX has to be obtained from other organisms or directly from the environment. Here, we report evidence that the source of TTX for toxic fish juveniles including the pufferfish (Chelonodon patoca) and the goby (Yongeichthys criniger) is in the food organisms, as seen in their gut contents. Next generation sequencing analysis for the mitochondrial COI gene showed that the majority of the sequence recovered from intestinal contents of these toxic fishes belonged to the flatworm Planocera multitentaculata, a polyclad flatworm containing highly concentrated TTX from the larval stage. PCR specific to P. multitentaculata also showed that DNA encoding the planocerid COI gene was strongly detected in the intestinal contents of the goby and pufferfish juveniles. Additionally, the planocerid specific COI sequence was detected in the environmental seawater collected from the water around the sampling locations for TTX-bearing fish. These results suggest that planocerid larvae are the major TTX supplier for juveniles of TTX-bearing fish species.

リンク情報
DOI
https://doi.org/10.1016/j.chemosphere.2020.126217
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32088461
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-85079641752&origin=inward
Scopus
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