- SOC BIOSCIENCE BIOENGINEERING JAPAN
We report a method for efficient mutagenesis of DNA in large vectors without subcloning. Two segments of the target DNA sequence, one having a mutation introduced via a mutant primer, were amplified by PCR and then the purified fragments were ligated to a vector. The mutation efficiency was nearly 100%. (C) 2008, The Society for Biotechnology. Japan. All rights reserved.
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