MISC

2009年1月

Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer

JOURNAL OF BIOSCIENCE AND BIOENGINEERING
  • Yu Kato
  • ,
  • Noriaki Arakawa
  • ,
  • Yusuke Masuishi
  • ,
  • Hiroshi Kawasaki
  • ,
  • Hisashi Hirano

107
1
開始ページ
95
終了ページ
97
記述言語
英語
掲載種別
DOI
10.1016/j.jbiosc.2008.09.003
出版者・発行元
SOC BIOSCIENCE BIOENGINEERING JAPAN

We report a method for efficient mutagenesis of DNA in large vectors without subcloning. Two segments of the target DNA sequence, one having a mutation introduced via a mutant primer, were amplified by PCR and then the purified fragments were ligated to a vector. The mutation efficiency was nearly 100%. (C) 2008, The Society for Biotechnology. Japan. All rights reserved.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.jbiosc.2008.09.003
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000263531300019&DestApp=WOS_CPL

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