MISC

2009年4月

Isoelectric focusing of high-molecular-weight protein complex under native conditions using agarose gel

ANALYTICAL BIOCHEMISTRY
  • Ryo Yokoyama
  • ,
  • Yuko Iwafune
  • ,
  • Hiroshi Kawasaki
  • ,
  • Hisashi Hirano

387
1
開始ページ
60
終了ページ
63
記述言語
英語
掲載種別
DOI
10.1016/j.ab.2009.01.009
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

The isolation and characterization of protein complexes are essential steps toward understanding cellular functions. A method for separating and characterizing high-molecular-weight protein complexes using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with native agarose gel isoelectric focusing (IEF) is described. Using this method, fractions containing high-molecular-weight protein complexes were analyzed. The advantages of using native agarose gel IEF include the ability to concentrate the protein complexes and the ease of handling when performing 2D separations. Although limited with respect to the size of molecules and particles that may be separated, this method is useful for the isolation and characterization of high-molecular-weight protein complexes. (C) 2009 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.ab.2009.01.009
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000264211600007&DestApp=WOS_CPL

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