MISC

2010年9月

Subcellular localization of the interaction of bipolar landmarks Bud8p and Bud9p with Rax2p in Saccharomyces cerevisiae diploid cells

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Yu Kato
  • ,
  • Hiroshi Kawasaki
  • ,
  • Noriaki Arakawa
  • ,
  • Hisashi Hirano

399
4
開始ページ
525
終了ページ
530
記述言語
英語
掲載種別
DOI
10.1016/j.bbrc.2010.07.102
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

In Saccharomyces cerevisiae, the bud site selection of diploid cells is regulated by at least four persistent landmarks, Bud8p, Bud9p, Rax1p, and Rax2p. Bud8p and Bud9p are essential for the establishment of bipolar budding and localize mainly to the distal and the proximal poles, respectively. Their subcellular localizations are regulated through interaction with Rax1p/Rax2p. We investigated when and where Bud8p and Bud9p physically interact with Rax2p in vivo using a split-GFP method. GFP fluorescence showed that Bud8p physically interacted with Rax2p at the proximal or distal pole in unbudded cells; a physical interaction was also observed at the opposite pole to the growing bud in mother cells with a large-size bud. Bud9p physically interacted with Rax2p at the birth scar in budded mother cells. These observations suggest that the interaction of Rax2p with Bud8p and Bud9p may contribute to the translocation of bipolar landmarks to the correct sites. (C) 2010 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2010.07.102
CiNii Articles
http://ci.nii.ac.jp/naid/80021247891
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20678480
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000281840200011&DestApp=WOS_CPL

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