MISC

2004年1月

Global gene repression by KaiC as a master process of prokaryotic circadian system

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Y Nakahira
  • ,
  • M Katayama
  • ,
  • H Miyashita
  • ,
  • S Kutsuna
  • ,
  • H Iwasaki
  • ,
  • T Oyama
  • ,
  • T Kondo

101
3
開始ページ
881
終了ページ
885
記述言語
英語
掲載種別
DOI
10.1073/pnas.0307411100
出版者・発行元
NATL ACAD SCIENCES

A kaiABC clock gene cluster was previously identified from cyanobacterium Synechococcus elongatus PCC 7942, and the feedback regulation of kai genes was proposed as the core mechanism generating circadian oscillation. In this study, we confirmed that the Kai-based oscillator is the dominant circadian oscillator functioning in cyanobacteria. We probed the nature of this regulation and found that excess KaiC represses not only kaiBC but also the rhythmic components of all genes in the genome. This result strongly suggests that the KaiC protein primarily coordinates genomewide gene expression, including its own expression. We also found that a promoter derived from E. coli is feedback controlled by KaiC and restores the complete circadian rhythm in kaiBC-inactivated arrhythmic mutants, provided it can express kaiB and kaiC genes at an appropriate level. Unlike eukaryotic models, specific regulation of the kaiBC promoter is not essential for cyanobacterial circadian oscillations.

Web of Science ® 被引用回数 : 94

リンク情報
DOI
https://doi.org/10.1073/pnas.0307411100
CiNii Articles
http://ci.nii.ac.jp/naid/80016431997
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/14709675
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000188555400035&DestApp=WOS_CPL