MISC

2005年9月

Transcriptional regulation of the circadian clock operon kaiBC by upstream regions in cyanobacteria

MOLECULAR MICROBIOLOGY
  • S Kutsuna
  • ,
  • Y Nakahira
  • ,
  • M Katayama
  • ,
  • M Ishiura
  • ,
  • T Kondo

57
5
開始ページ
1474
終了ページ
1484
記述言語
英語
掲載種別
DOI
10.1111/j.1365-2958.2005.04781.x
出版者・発行元
BLACKWELL PUBLISHING

In the cyanobacterium, Synechococcus elongatus PCC 7942, the kaiBC operon is upregulated by the KaiA protein and downregulated by the KaiC protein to generate circadian oscillation. We investigated the regulation of kaiBC transcription. A primer extension and deletion analyses of the upstream region mapped the sufficient promoter region (SPR) to base pairs -55 to +1 (the transcription start site, TSS) and identified a constitutive negative regulatory region upstream of the SPR (base pairs -897 to -56) that extended into the coding sequence of kaiA. Base-pair substitution within the SPR identified a sequence from -52 to -28 that was the essential element for transcription. Most of the examined sequences drove rhythmic expression of a luxAB reporter that was similar to the expression driven by the kaiBC promoter (PkaiBC) and responded to the overexpression of kaiA or kaiC, even in a promoter activity range of 1-8000%. These results indicate that circadian feedback regulation by KaiA and KaiC is addressed to a global step preceding transcription driven by PkaiBC. However, increasing or decreasing the intrinsic activity of PkaiBC greatly affected the rhythm, suggesting that constitutive adjustment of PkaiBC activity by the sequences identified here is essential for the oscillator.

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1111/j.1365-2958.2005.04781.x
CiNii Articles
http://ci.nii.ac.jp/naid/80017774945
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16102014
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000231021600022&DestApp=WOS_CPL