2014年4月
Effects of Cryopreservation on the Cell Viability, Proliferative Capacity and Neuronal Differentiation Potential of Canine Bone Marrow Stromal Cells
JOURNAL OF VETERINARY MEDICAL SCIENCE
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- 巻
- 76
- 号
- 4
- 開始ページ
- 573
- 終了ページ
- 577
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1292/jvms.13-0296
- 出版者・発行元
- JAPAN SOC VET SCI
We investigated the cell viability, proliferative capacity and neuronal differentiation potential of canine bone marrow stromal cells (BMSCs) after cryopreservation. BMSCs were cryopreserved using cryoprotectant solutions with 10% DMSO and 10% FBS (DF group) or without DMSO and FBS (DF-free group); fresh BMSCs were used as a control. The cell viability and proliferative capacity of BMSCs were similar in the DF-free and control groups, while those in the DF group were lower. In all groups, BMSCs differentiated into neuron-like cells that stained positive against neuron markers, and the mRNA expression levels of neuron markers increased after neuronal induction. In conclusion, cryopreservation with DF-free cryoprotectant solution did not diminish the cell viability, proliferative capacity or neuronal differentiation potential of canine BMSCs.
- リンク情報
- ID情報
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- DOI : 10.1292/jvms.13-0296
- ISSN : 0916-7250
- eISSN : 1347-7439
- Web of Science ID : WOS:000335207800016