2015年12月
First Isolation of Azole-Resistant Cryptococcus neoformans from Feline Cryptococcosis
MYCOPATHOLOGIA
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- 巻
- 180
- 号
- 5-6
- 開始ページ
- 427
- 終了ページ
- 433
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/s11046-015-9919-9
- 出版者・発行元
- SPRINGER
We report here, to the best of our knowledge, the first description of an in vitro fluconazole (FLZ)-resistant Cryptococcus neoformans var. grubii from a case of feline cryptococcosis. In vitro testing demonstrated that this isolate was resistant to FLZ (minimum inhibitory concentration, MIC, of 128 mu g/ml) but remained susceptible to amphotericin B (0.064 A mu g/ml), itraconazole (0.38 A mu g/ml), voriconazole (0.023 A mu g/ml), and posaconazole (0.125 A mu g/ml). The predicted amino acid sequence of the lanosterol 14-alpha demethylase (ERG11) protein in the isolate was identical to that of the C. neoformans var. grubii reference strain, indicating that resistance was not mediated by mutation of the target gene's open reading frame. The RT-qPCR analysis for ERG11 and ATP-binding cassette (ABC) transporter-encoding gene (AFR1) indicated that the isolate increased transcription factor function of ERG11 and AFR1 than that of FLZ-susceptive strains. This observation, in combination with the lack of resistance to other azoles (that is, lack of crossresistance), suggests that resistance in our isolate was the result of overexpression of the endogenous ERG11 and ABC transporter.
- リンク情報
- ID情報
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- DOI : 10.1007/s11046-015-9919-9
- ISSN : 0301-486X
- eISSN : 1573-0832
- Web of Science ID : WOS:000365183400018