MISC

査読有り
2015年12月

First Isolation of Azole-Resistant Cryptococcus neoformans from Feline Cryptococcosis

MYCOPATHOLOGIA
  • Rui Kano
  • ,
  • Miki Okubo
  • ,
  • Tokuma Yanai
  • ,
  • Atsuhiko Hasegawa
  • ,
  • Hiroshi Kamata

180
5-6
開始ページ
427
終了ページ
433
記述言語
英語
掲載種別
DOI
10.1007/s11046-015-9919-9
出版者・発行元
SPRINGER

We report here, to the best of our knowledge, the first description of an in vitro fluconazole (FLZ)-resistant Cryptococcus neoformans var. grubii from a case of feline cryptococcosis. In vitro testing demonstrated that this isolate was resistant to FLZ (minimum inhibitory concentration, MIC, of 128 mu g/ml) but remained susceptible to amphotericin B (0.064 A mu g/ml), itraconazole (0.38 A mu g/ml), voriconazole (0.023 A mu g/ml), and posaconazole (0.125 A mu g/ml). The predicted amino acid sequence of the lanosterol 14-alpha demethylase (ERG11) protein in the isolate was identical to that of the C. neoformans var. grubii reference strain, indicating that resistance was not mediated by mutation of the target gene's open reading frame. The RT-qPCR analysis for ERG11 and ATP-binding cassette (ABC) transporter-encoding gene (AFR1) indicated that the isolate increased transcription factor function of ERG11 and AFR1 than that of FLZ-susceptive strains. This observation, in combination with the lack of resistance to other azoles (that is, lack of crossresistance), suggests that resistance in our isolate was the result of overexpression of the endogenous ERG11 and ABC transporter.

Web of Science ® 被引用回数 : 4

リンク情報
DOI
https://doi.org/10.1007/s11046-015-9919-9
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365183400018&DestApp=WOS_CPL