論文

査読有り 国際誌
2020年5月

Neuroprotective Effects of Endogenous Secretory Receptor for Advanced Glycation End-products in Brain Ischemia.

Aging and disease
  • Yu Shimizu
  • ,
  • Ai Harashima
  • ,
  • Seiichi Munesue
  • ,
  • Masahiro Oishi
  • ,
  • Tsuyoshi Hattori
  • ,
  • Osamu Hori
  • ,
  • Yasuko Kitao
  • ,
  • Hiroshi Yamamoto
  • ,
  • Nontaphat Leerach
  • ,
  • Mitsutoshi Nakada
  • ,
  • Yasuhiko Yamamoto
  • ,
  • Yasuhiko Hayashi

11
3
開始ページ
547
終了ページ
558
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.14336/AD.2019.0715

The receptor for advanced glycation end-products (RAGE) is expressed on human brain endothelial cells (HBEC) and is implicated in neuronal cell death after ischemia. We report that endogenous secretory RAGE (esRAGE) is a splicing variant form of RAGE that functions as a decoy against ischemia-induced neuronal cell damage. This study demonstrated that esRAGE was associated with heparan sulphate proteoglycans on HBEC. The parabiotic experiments between human esRAGE overexpressing transgenic (Tg), RAGE knockout (KO), and wild-type (WT) mice revealed a significant neuronal cell damage in the CA1 region of the WT side of parabiotic WT→WT mice, but not of Tg→WT mice, 7 days after bilateral common carotid artery occlusion. Human esRAGE was detected around the CA1 neurons in the WT side of the parabiotic Tg→WT pair, but not in the KO side of the Tg→KO pair. To elucidate the dynamic transfer of esRAGE into the brain, we used the blood-brain barrier (BBB) system (PharmaCo-Cell) with or without RAGE knockdown in endothelial cells. A RAGE-dependent transfer of esRAGE was demonstrated from the vascular to the brain side. These findings suggested that esRAGE is associated with heparan sulphate proteoglycans and is transferred into the brain via BBB to exert its neuroprotective effects in ischemia.

リンク情報
DOI
https://doi.org/10.14336/AD.2019.0715
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32489701
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220285
ID情報
  • DOI : 10.14336/AD.2019.0715
  • PubMed ID : 32489701
  • PubMed Central 記事ID : PMC7220285

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