論文

査読有り 国際誌
2005年4月1日

Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9.

Genes & development
  • Makoto Tachibana
  • ,
  • Jun Ueda
  • ,
  • Mikiko Fukuda
  • ,
  • Naoki Takeda
  • ,
  • Tsutomu Ohta
  • ,
  • Hiroko Iwanari
  • ,
  • Toshiko Sakihama
  • ,
  • Tatsuhiko Kodama
  • ,
  • Takao Hamakubo
  • ,
  • Yoichi Shinkai

19
7
開始ページ
815
終了ページ
26
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1101/gad.1284005
出版者・発行元
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT

Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin. GLP-deficiency led to embryonic lethality, a severe reduction of H3-K9 mono- and dimethylation, the induction of Mage-a gene expression, and HP1 relocalization in embryonic stem cells, all of which were phenotypes of G9a-deficiency. Furthermore, we show that G9a and GLP formed a stoichiometric heteromeric complex in a wide variety of cell types. Biochemical analyses revealed that formation of the G9a/GLP complex was dependent on their enzymatic SET domains. Taken together, our new findings revealed that G9a and GLP cooperatively exert H3-K9 methyltransferase function in vivo, likely through the formation of higher-order heteromeric complexes.

リンク情報
DOI
https://doi.org/10.1101/gad.1284005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15774718
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1074319
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000228154100007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1101/gad.1284005
  • ISSN : 0890-9369
  • PubMed ID : 15774718
  • PubMed Central 記事ID : PMC1074319
  • Web of Science ID : WOS:000228154100007

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