論文

査読有り 筆頭著者 責任著者
2011年11月

Gene Expression Profiling of Neural Stem Cells and Identification of Regulators of Neural Differentiation During Cortical Development

STEM CELLS
  • Toshiyuki Ohtsuka
  • ,
  • Hiromi Shimojo
  • ,
  • Mitsuhiro Matsunaga
  • ,
  • Naoki Watanabe
  • ,
  • Kohei Kometani
  • ,
  • Nagahiro Minato
  • ,
  • Ryoichiro Kageyama

29
11
開始ページ
1817
終了ページ
1828
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/stem.731
出版者・発行元
WILEY-BLACKWELL

During mammalian brain development, neural stem cells transform from neuroepithelial cells to radial glial cells and finally remain as astrocyte-like cells in the postnatal and adult brain. Neuroepithelial cells divide symmetrically and expand the neural stem cell pool; after the onset of neurogenesis, radial glial cells sequentially produce deep layer neurons and then superficial layer neurons by asymmetric, self-renewing divisions during cortical development. Thereafter, gliogenesis supersedes neurogenesis, while a subset of neural stem cells retain their stemness and lurk in the postnatal and adult brain. Thus, neural stem cells undergo alterations in morphology and the capacity to proliferate or give rise to various types of neural cells in a temporally regulated manner. To shed light on the temporal alterations of embryonic neural stem cells, we sorted the green fluorescent protein-positive cells from the dorsolateral telencephalon (neocortical region) of pHes1-d2EGFP transgenic mouse embryos at different developmental stages and performed gene expression profiling. Among dozens of transcription factors differentially expressed by cells in the ventricular zone during the course of development, several of them exhibited the activity to inhibit neuronal differentiation when overexpressed. Furthermore, knockdown of Tcf3 or Klf15 led to accelerated neuronal differentiation of neural stem cells in the developing cortex, and neurospheres originated from Klf15 knockdown cells mostly lacked neurogenic activities and only retained gliogenic activities. These results suggest that Tcf3 and Klf15 play critical roles in the maintenance of neural stem cells at early and late embryonic stages, respectively. STEM CELLS 2011;29:1817-1828

リンク情報
DOI
https://doi.org/10.1002/stem.731
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201202298671233561
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21898698
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000296565500018&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/stem.731
  • ISSN : 1066-5099
  • J-Global ID : 201202298671233561
  • PubMed ID : 21898698
  • Web of Science ID : WOS:000296565500018

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