2002年11月
Diversity of S-RNase genes and S-haplotypes in Japanese plum (Prunus salicina Lindl.)
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
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- 巻
- 77
- 号
- 6
- 開始ページ
- 658
- 終了ページ
- 664
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- HEADLEY BROTHERS LTD
This report demonstrates the diversity of S-haplotypes in Japanese plum by molecular, cloning of genomic DNAs and cDNAs that encode S-RNases. Nine different DNA fragments, designated as S-S', were obtained from 17 Japanese plum cultivars by PCR with an S-RNase gene-specific primer set, Pru-C2 and PCE-R. Eleven different S-haplotypes were found in these cultivars. The banding patterns obtained with another S-RNase gene-specific primer set, Pru-T2 and PCE-R, corresponded to the S-haplotypes predicted from the Pru-C2 and PCE-R primer set. Several cultivars had the same S-haplotypes. Partial genomic DNAs for eight S-RNase genes and cDNAs for two S-RNases were cloned and sequenced. Deduced amino acid sequences contained conserved regions among the rosaceous S-RNases. Comparisons of the-sequences from cDNAs and genomic DNAs revealed the presence of two introns in the S-RNase genes of Japanese plum as in other Prunus S-RNase genes. Pollination incompatibility groups and self-compatibility in Japanese plum were discussed with reference to the S-haplotypes.
- リンク情報
- ID情報
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- ISSN : 0022-1589
- Web of Science ID : WOS:000180058900003