2000年
Immunolocalization of glucomannans in the cell wall of differentiating tracheids in Chamaecyparis obtusa
PROTOPLASMA
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- 巻
- 213
- 号
- 3-4
- 開始ページ
- 148
- 終了ページ
- 156
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- SPRINGER-VERLAG WIEN
A polyclonal antibody against glucomannans (GMs) was raised in a mouse. A dot-blot immunoassay and competitive-inhibition tests indicated that the antibody was specific for GMs. The antibody enables visualization of the localization of GMs in differentiating tracheids of Chamaecyparis obtusa. Labeling of GMs was restricted to the secondary walls of the tracheids. The labeling density temporarily increased and then decreased in the outer and middle layers of the secondary wall during cell wall formation. This ii probably due to the accumulation of lignin. In comparison with previous studies of glucuronoxylans, there must be a clear difference between the deposition of GMs and that of glucuronoxylans.
- リンク情報
- ID情報
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- ISSN : 0033-183X
- Web of Science ID : WOS:000089680700004