2000年8月
Dominant negative isoform of the Ikaros gene in patients with adult B-cell acute lymphoblastic leukemia
CANCER RESEARCH
- 巻
- 60
- 号
- 15
- 開始ページ
- 4062
- 終了ページ
- 4065
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- AMER ASSOC CANCER RESEARCH
Gene targeting studies in mice have shown that the transcription factor Ikaros plays an essential role in lymphoid development and as a tumor suppressor in T cells, whereas the related gene Aiolos functions as a tumor suppressor in B cells. We analyzed the expression levels of the Ikaros gene family, Ikaros and Aiolos, in human bone marrow samples from patients with adult acute lymphoblastic leukemia [ALL (n = 46; B-cell ALL = 41; T-cell ALL = 5)]. Overexpression of the dominant negative isoform of Ikaros gene Ik-6 was observed in 14 of 41 B-cell ALL patients by reverse transcription-PCR, and the results were confirmed by sequencing analysis and immunoblotting, None of the other dominant negative isoforms of the Ikaros gene were detected by reverse transcription-PCR analysis, Southern blotting analysis with PstI digestion revealed that those patients with the dominant negative isoform Ik-6 might have small mutations in the Ikaros locus. We did not detect any overexpression of dominant negative isoforms of Aiolos in adult ALL patients. These results suggest that Ikaros plays a key role in human B-cell malignancies through the dominant negative Isoform Ik-6.
- リンク情報
- ID情報
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- ISSN : 0008-5472
- Web of Science ID : WOS:000088625700016