1996年
Involvement of Rabphilin-3a in cortical granule exocytosis in mouse eggs
Journal of Cell Biology
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- 巻
- 135
- 号
- 6
- 開始ページ
- 1741
- 終了ページ
- 1747
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1083/jcb.135.6.1741
Rabphilin-3A is a putative target protein for Rab3A, a member of the small GTP-binding protein superfamily that has been suggested to play a role in regulated exocytosis in presynapses. In this study we determined the expression and the function of Rabphilin-3A in mouse eggs at fertilization. Rabphilin-3A mRNA and protein were detected by reverse transcriptase-PCR and immunoblot analysis, respectively, in metaphase II mouse eggs. Immunofluorescence analysis showed that Rabphilin-3A protein was distributed in the cortical region in eggs. Sperm induces cortical granule (CG) exocytosis via an increase in cytosolic Ca2+ at fertilization. We microinjected the NH2- or COOH-terminal fragment of recombinant Rabphilin- 3A into metaphase II eggs. Neither treatments altered the sperm-induced cytosolic Ca2+ increase, but both inhibited CG exocytosis in a dose- dependent manner. The NH2-terminal fragment was more effective than the COOH-terminal fragment. Full-length Rabphilin-3A did not affect CG exocytosis, but it attenuated the inhibition of CG exocytosis by the NH2- terminal fragment. These results show that Rabphilin-3A is involved in Ca2+-dependent CG exocytosis at fertilization in mouse eggs.
- リンク情報
- ID情報
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- DOI : 10.1083/jcb.135.6.1741
- ISSN : 0021-9525
- CiNii Articles ID : 80009434192
- PubMed ID : 8991087
- SCOPUS ID : 0030461545