Analysis and enhancement of the ethanol resistance of Pichia kudriavzevii N77-4, a strain newly isolated from the Korean traditional fermentation starter Nuruk, for improved fermentation performance
Sugiyama M, Fukaya T, Moon JY, Ishida K, Matsushita A, Kim SY, Yeo SH
J. Phys.: Conf. Ser. in press Feb 2019 [Refereed]
Overexpression of PkINO1 improves ethanol resistance of Pichia kudriavzevii N77-4 isolated from the Korean traditional fermentation starter nuruk
Sugiyama M, Baek SY, Takashima S, Miyashita N, Ishida K, Mun J, Yeo SH
J Biosci Bioeng. 126(6) 682-689 Dec 2018 [Refereed]
Sasano Y, Sakata T, Okusaki S, Sugiyama M, Kaneko Y, Harashima S
Genes Genet. Syst. Nov 2018 [Refereed]
Molecular breeding of Saccharomyces cerevisiae with high RNA content by harnessing essential ribosomal RNA transcription regulator
AMB Express 7 32 Dec 2017 [Refereed]
Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation
Kitichantaropas Y, Boonchird C, Sugiyama M, Kaneko Y, Harashima S, Auesukaree C
AMB Express 6 107 Dec 2016 [Refereed]
Overexpression of ESBP6 improves lactic acid resistance and production in Saccharomyces cerevisiae
Sugiyama, M., Akase, S., Nakanishi, R., Kaneko, Y., Harashima, S.
J. Biosci. Bioeng. 122(4) 415-420 Oct 2016 [Refereed]
CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae
Sasano, Y., Nagasawa. K., Kaboli. S., Sugiyama, M., Harashima, S.
Scientific Reports 6 30278 Aug 2016 [Refereed]
Improved stress resistance and ethanol production by segmental haploidization of the diploid genome in Saccharomyces cerevisiae
Kaboli, S., Miyamoto, T., Sunada, K., Sasano, Y., Sugiyama, M., Harashima, S.
J. Biosci. Bioeng. 121(6) 638-644 Jun 2016 [Refereed]
Candida tropicalis Isolated from Tuak, a North Sumatera-Indonesian Traditional Beverage, for Bioethanol Production
Hermansyah, Novia, Sugiyama, M., Harashima, S.
Microbiol. Biotechnol. Lett. 43(3) 241-248 Oct 2015 [Refereed]
Genome-wide construction of a series of designed segmental aneuploids in Saccharomyces cerevisiae
Natesuntorn, W., Iwami, K., Matsubara, Y., Sasano, Y., Sugiyama, M., Kaneko ,Y., Harashima, S.
Scientific Reports 5 12510 Sep 2015 [Refereed]
Stabilization of mini-chromosome segregation during mitotic growth by overexpression of YCR041W and its application to chromosome engineering in Saccharomyces cerevisiae
Sasano, Y., Yamagishi, K., Tanikawa, M., Nakazawa, T., Sugiyama, M., Kaneko, Y., Harashima, S.
J. Biosci. Bioeng. 119(5) 526-531 May 2015 [Refereed]
Type 2C protein phosphatase Ptc6 participates in activation of the Slt2-mediated cell wall integrity pathway in Saccharomyces cerevisiae
Sharmin, D., Sasano, Y., Sugiyama, M., Harashima, S.
J. Biosci. Bioeng. 119(4) 392-398 Apr 2015 [Refereed]
Nuclear localization domains of GATA activator Gln3 are required for transcription of target genes through dephosphorylation in Saccharomyces cerevisiae
Numamoto, M., Tagami, S., Ueda, Y., Imabeppu, U., Sasano, Y, Hirasaki, M., Sugiyama, M., Maekawa, H., Harashima, S.
J. Biosci. Bioeng. 120(2) 121-127 Jan 2015 [Refereed]
The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiae
Numamoto, M., Sasano, Y, Hirasaki, M., Sugiyama, M., Maekawa, H., Harashima, S.
J. Biochem. 157(1) 53-64 Jan 2015 [Refereed]
Effects of deletion of different PP2C protein phosphatase genes on stress responses in Saccharomyces cerevisiae
Sharmin, D., Sasano, Y., Sugiyama, M., Harashima, S.
Yeast 31(10) 393-409 Oct 2014 [Refereed]
Genome-wide mapping of unexplored essential regions in the S. cerevisiae genome: Evidence for hidden synthetic lethal combinations in a genetic interaction network
Kaboli S., Yamakawa T., Sunada K., Takagaki T., Sasano Y., Sugiyama M., Kaneko Y., Harashima S.
Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae
Sugiyama, M., Akase, SP., Nakanishi, R., Horie, H., Kaneko, Y., Harashima, S.
Appl. Environ. Microbiol. 80(11) 3488-3495 Jun 2014 [Refereed]
Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Deltamsg5Delta double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1
Laviña, WA, Shahsavarani H, Saidi A, Sugiyama M, Kaneko Y, Harashima S.
J. Biosci. Bioeng. 117(2) 135-141 Feb 2014 [Refereed]
Cloning and functional analysis of HpFAD2 and HpFAD3 genes encoding Δ12- and Δ15-fatty acid desaturases in Hansenula polymorpha
Sangwallek J, Kaneko Y, Tsukamoto T, Marui M, Sugiyama M, Ono H, Bamba T, Fukusaki E, Harashima S.
Gene 533(1) 110-118 Jan 2014 [Refereed]
Ketoacyl synthase domain is a major determinant for fatty acyl chain length in Saccharomyces cerevisiae
Sangwallek J, Kaneko Y, Sugiyama M, Ono H, Bamba T, Fukusaki E, Harashima S.
Arch. Microbiol. 195(12) 843-852 Dec 2013 [Refereed]
Increase in rRNA content in a Saccharomyces cerevisiae suppressor strain from rrn10 disruptant by rDNA cluster duplication
Khatun K, Sasano Y, Sugiyama M, Kaneko Y, Harashima S.
Appl. Microbiol. Biotechnol. 97(20) 9011-9019 Oct 2013 [Refereed]
Khatun F, Kurata K, Chuwattanakul V, Sugiyama M, Kaneko Y, Harashima, S
J. Biosci. Bioeng. 116(4) 423-432 Oct 2013 [Refereed]
Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae
Suzuki T, Sakamoto T, Sugiyama M, Ishida N, Kambe H, Obata S, Kaneko .Y, Takahashi H, Harashima S
J. Biosci. Bioeng. 115(5) 467-474 May 2013 [Refereed]
Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration
Laviña W., Hermansya.h, Sugiyama M, Kaneko Y, Harashima S
J. Biosci. Bioeng. 115(2) 138-146 Feb 2013 [Refereed]
Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14
Shahsavarani H, Hasegawa D, Yokota D, Sugiyama M, Kaneko Y, Boonchird C, Harashima S
J. Biosci. Bioeng. 115(1) 20-23 Jan 2013 [Refereed]
Shahsavarani H, Sugiyama M, Kaneko Y, Chuenchit B, Harashima S
Biotechnol. Adv. 30(6) 1289-1300 Nov 2012 [Refereed][Invited]
Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits
Auesukaree C, Koedrith P, Saenpayavai P, Asvarak T, Benjaphokee S, Sugiyama M, Kaneko Y, Harashima S, Boonchird C
J. Biosci. Bioeng. 114(2) 144-149 Aug 2012 [Refereed]
Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning
Chuwattanakul V, Sugiyama M, Khatun F, Kurata K, Tomita I, Kaneko Y, Harashima S.
J. Biosci. Bioeng. 114(1) 17-22 Jul 2012 [Refereed]
Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes
Ueda Y, Ikushima S, Sugiyama M, Matoba R, Kaneko Y, Matsubara K, Harashima S.
J. Biosci. Bioeng. 113(6) 675-682 Jun 2012 [Refereed]
Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae
Suzuki T, Sugiyama M, Wakazono K, Kaneko Y, Harashima S
J. Biosci. Bioeng. 113(4) 421-430 Apr 2012 [Refereed]
Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol
Benjaphokee S, Hasegawa D, Yokota D, Asvarak T, Auesukaree C, Sugiyama M, Kaneko Y, Boonchird C, Harashima S
NewBiotechnology 29(3) 379-386 Feb 2012 [Refereed]
Creation of an ethanol-tolerant yeast strain by genome reconstruction based on chromosome splitting technology
Park, A. H., Sugiyama, M., Harashima, S., Kim, Y. H.
J. Microbiol. Biotechnol. 22(2) 184-189 Feb 2012 [Refereed]
CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae
Benjaphokee S, Koedrith P, Auesukaree C, Asvarak T, Sugiyama M, Kaneko Y, Boonchird C, Harashima S
NewBiotechnology 29(2) 166-176 Jan 2012 [Refereed]
Construction of a Saccharomyces cerevisiae strain with a high level of RNA
Chuwattanakul V, Kim YH, Sugiyama M, Nishiuchi H, Miwa H, Kaneko Y, Harashima S
J. Biosci. Bioeng. 112(1) 1-7 Jul 2011 [Refereed]
Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae
Sugiyama M, Nugroho S, Iida N, Sakai T, Kaneko Y, Harashima S
Chromosome rearrangements, especially chromosomal deletions, have been exploited as important resources for functional analysis of genomes. To facilitate this analysis, we applied a previously developed method for chromosome splitting for the dir...
Appl. Microbiol. Biotechnol. 79(4) 699-706 Jun 2008 [Refereed]
A novel chromosome engineering technology is described which enables conditional splitting of natural chromosomes in haploid cells of the yeast Saccharomyces cerevisiae. The technology consists of introduction of a recognition sequence for the hom...
Analysis and improvement of ethanol resistance and fermentation of Pichia kudriavzevii N77-4 isolated from the Korean traditional fermentation starter Nuruk
Stress Biology of Yeasts and Fungi: Application for Industrial Brewing and Fermentation
Kitagaki, H. and Takagi, H. (Eds.) (Part:Contributor, Sugiyama, M., Sasano, Y., Harashima, S., Adaptation mechanism of yeast to weak organic acid (p.107-121))
Springer Japan Mar 2015
Microbial Production: From Genome Design to Cell Engineering
Anazawa, H. and Shimizu, S (Part:Contributor, Sasano Y, Sugiyama M, Harashima S., Development and application of novel genome engineering technologies in Saccharomyces cerevisiae (p.53-62))
Springer Japan Mar 2014 ISBN:978-4-431-546061-1
Methods in Molecular Biology: YAC Protocols, second edition
MacKenzie, A. (Ed.) (Part:Contributor, Kim YH, Sugiyama M, Kaneko Y, Fukui K, Kobayashi A, Harashima S, 10A: polymerase chain reaction-mediated yeast artificial chromosome-splitting technology for generating targeted yeast artificial chromosomes subclones (p.103-115))
45th Annual Meeting and International Symposium of the Korean Society for Microbiology and Biotechnology 28 Jun 2018
Characterization and improvement of ethanol resistance of Pichia kudriavzevii N77-4 isolated from the Korean traditional fermentation starter Nuruk[Invited]
Sugiyama, M.
Research Seminar in National Institute of Agricultural Science, Rural Development Administration, Korea 26 Apr 2018
bio.eng.osaka-u.ac.jp