論文

2006年10月

Tandem and single genes of three membrane-bound nitrate transporters in the nar gene cluster of the moderately halophilic denitrifier, Halomonas halodenitrificans

DNA SEQUENCE
  • Nobuhiko Sakurai
  • ,
  • Azumi Asada
  • ,
  • Shuhei Mano
  • ,
  • Kunishige Kataoka
  • ,
  • Takeshi Sakurai

17
5
開始ページ
363
終了ページ
369
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/10425170601017202
出版者・発行元
TAYLOR & FRANCIS LTD

We identified the genes encoding the membrane-bound nitrate reductase (Nar) from the moderate halophile, Halomonas halodenitrificans, and examined the structure of the gene cluster. Screening of a H. halodenitrificans genomic DNA library in X EMBL3 phage by chromosome walking revealed that the region adjacent to the nor gene cluster encoding nitric oxide (NO) reductase contains three nitrate transporters: tandem narK2 and narK1.1 genes and a single narK1.2 gene encoded in opposite directions. NarK1.1 and NarK1.2 proteins, which have 12 putative membrane-spanning helices, were classified as type I NarK, whereas NarK2, which has 14 putative membrane-spanning helices, was classified as a type II NarK. NarK1.1 and NarK2 proteins were considered to be functionally and structurally linked in the cytoplasmic membrane. The systems regulating the expression of the tandem narK2K1.1 gene and the single narK1.2 gene were found to be different. Further, binding sites for NarL and Fnr-like proteins are present in the promoter region of the narK2 gene.

Web of Science ® 被引用回数 : 1

リンク情報
DOI
https://doi.org/10.1080/10425170601017202
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000243257700007&DestApp=WOS_CPL

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