J-GLOBAL         Last updated: Sep 28, 2018 at 17:39
Kindai University
Faculty of Pharmacy Department of Pharmaceutical Sciences
Job title
Associate Professor

Research Interests


Research Areas



Faculty of Pharmacentical Sciences, Kinki University
Faculty of Pharmaceutical Science, Kinki University

Published Papers

Electrophoretic Approach for Biopharmaceutical Research and Development
Journal of the Mass Spectrometry Society of Japan   66(4) 154-160   Aug 2018
Simultaneous Analysis of Sulfated and Phosphorylated Glycans by Serotonin-Immobilized Column Enrichment and Hydrophilic Interaction Chromatography.
Yamada K, Kayahara H, Kinoshita M, Suzuki S.
90(14) 8387-8395   Jul 2018   [Refereed]
Yamamoto S, Himeno M, Kobayashi M, Akamatsu M, Satoh R, Kinoshita M, Sugiura R, Suzuki S.
Analyst   142(18) 3216-3423   Sep 2017   [Refereed]
Yamada H, Matsumura C, Yamada K, Teshima K, Hiroshima T, Kinoshita M, Suzuki S, Kakehi K.
Electrophoresis   38(9-10) 1344-1352   May 2017   [Refereed]
Yamagami M, Matsui Y, Hayakawa T, Yamamoto S, Kinoshita M, Suzuki S.
J.Chromatogr.A   1416 157-162   May 2017   [Refereed]
Yamamoto S, Kinoshita M, Suzuki S.
J Pharm Biomed Anal.   25(130) 273-300   Oct 2016   [Refereed]
Takeda Y, Hayashi Y, Utamura N, Takamoto C, Kinoshita M, Yamamoto S, Hayakawa T, Suzuki S.
J Chromatogr A.   1427 170-176   Jan 2016   [Refereed]
Yamamoto S, Tamata Y, Sejima K, Kinoshita M, Suzuki S
Analytical and bioanalytical chemistry   407(20) 6201-6206   Aug 2015   [Refereed]
Yamamoto S, Nagai E, Asada Y, Kinoshita M, Suzuki S
Analytical and bioanalytical chemistry   407(5) 1499-1503   Feb 2015   [Refereed]
Higashi K, Asano K, Yagi M, Yamada K, Arakawa T, Ehashi T, Mori T, Sumida K, Kushida M, Ando S, Kinoshita M, Kakehi K, Tachibana T, Saito K
The Journal of biological chemistry   289(37) 25833-25843   Sep 2014   [Refereed]
Iwatsuka K, Iwamoto H, Kinoshita M, Inada K, Yasueda S, Kakehi K
Current eye research   39(7) 686-694   Jul 2014   [Refereed]
Kinoshita M, Mitsui Y, Kakoi N, Yamada K, Hayakawa T, Kakehi K
Journal of proteome research   13(2) 1021-1033   Feb 2014   [Refereed]
Kinoshita M, Kakehi K
Methods in molecular biology (Clifton, N.J.)   1200 131-146   2014   [Refereed]
Yamada K, Kinoshita M, Jo Y, Inoue T, Aoshima M, Hasegawa K, Sei K, Kita S, Kakehi K
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan   134(11) 1209-1217   2014   [Refereed]
Kinoshita M, Nakatsuji Y, Suzuki S, Hayakawa T, Kakehi K
Journal of chromatography. A   1309 76-83   Sep 2013   [Refereed]
Iwatsuka K, Watanabe S, Kinoshita M, Kamisue K, Yamada K, Hayakawa T, Suzuki T, Kakehi K
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences   928 16-21   Jun 2013   [Refereed]
Mitsui Y, Yamada K, Hara S, Kinoshita M, Hayakawa T, Kakehi K
Journal of pharmaceutical and biomedical analysis   70 718-726   Nov 2012   [Refereed]
Maeda E, Kita S, Kinoshita M, Urakami K, Hayakawa T, Kakehi K
Analytical chemistry   84(5) 2373-2379   Mar 2012   [Refereed]
Yamada K, Mitsui Y, Kakoi N, Kinoshita M, Hayakawa T, Kakehi K
Analytical biochemistry   421(2) 595-606   Feb 2012   [Refereed]
Iwatsuka K, Yasueda S, Bando E, Fujii H, Terada T, Okubo H, Iwamoto H, Kinoshita M, Kakehi K
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences   879(27) 2866-2870   Oct 2011   [Refereed]
Kinoshita M, Kakoi N, Matsuno YK, Hayakawa T, Kakehi K
Biomedical chromatography : BMC   25(5) 588-593   May 2011   [Refereed]
Harazono A, Kobayashi T, Kawasaki N, Itoh S, Tada M, Hashii N, Ishii A, Arato T, Yanagihara S, Yagi Y, Koga A, Tsuda Y, Kimura M, Sakita M, Kitamura S, Yamaguchi H, Mimura H, Murata Y, Hamazume Y, Sato T, Natsuka S, Kakehi K, Kinoshita M, Watanabe S, Yamaguchi T
Biologicals : journal of the International Association of Biological Standardization   39(3) 171-180   May 2011   [Refereed]
Maeda E, Urakami K, Shimura K, Kinoshita M, Kakehi K
Journal of chromatography. A   1217(45) 7164-7171   Nov 2010   [Refereed]
Hiratsuka T, Matsuzaki S, Miyata S, Kinoshita M, Kakehi K, Nishida S, Katayama T, Tohyama M
PLoS One   5(10) e1328   Oct 2010   [Refereed]
Recently, several studies have reported Yokukansan (Tsumura TJ-54), a traditional Japanese medicine, as a potential new drug for the treatment of Alzheimer's disease (AD). Endoplasmic reticulum (ER) stress is known to play an important role in the...
Journal of chromatography. A   1217(45) 7164-71   Oct 2010
A robust and highly reproducible capillary isoelectric focusing (cIEF) method for the evaluation of charge heterogeneity of monoclonal antibody (mAb)
pharmaceutical which contains covalently bound antitumor compounds was developed using a combinat...
Yamada K, Hyodo S, Kinoshita M, Hayakawa T, Kakehi K
Analytical chemistry   82(17) 7436-7443   Sep 2010   [Refereed]
Analytical chemistry   82(17) 7436-7443   Jul 2010
We developed an automatic apparatus for the release of O-glycans from mucin-type glycoproteins and proteoglycans. The method allows rapid release of O-glycans as the reducing form within 10 min. In the present study, we connected the device to a M...
Anal Biochem.   362(2) 245-257   May 2010
An apparatus, AutoGlycoCutter (AGC), was developed as a tool for rapid release of O-linked-type glycans under alkaline conditions. This system allowed rapid release of oligosaccharides at the glycosaminoglycan-protein linkage region in proteoglyc...
Takada H, Nishida A, Domae M, Kita A, Yamano Y, Uchida A, Ishiwata S, Fang Y, Zhou X, Masuko T, Kinoshita M, Kakehi K, Sugiura R
Mol Biol Cell.   21(4) 674-685   Feb 2010   [Refereed]
Yamada K, Watanabe S, Kita S, Kinoshita M, Hayakawa T, Kakehi K
Analytical biochemistry   396(1) 161-163   Jan 2010   [Refereed]
Kamei N, Fukui R, Suzuki Y, Kajihara Y, Kinoshita M, Kakehi K, Hojo H, Tezuka K, Tsuji T
Biochem Biophys Res Commun   391(1) 557-563   Jan 2010   [Refereed]
Here we demonstrate the integral involvement of a specific N-glycan from amongst the three glycans present on inducible costimulator (ICOS), a T-cell costimulatory molecule, in proper protein folding and intracellular trafficking to the cell surfa...
Biomed Chromatogr      Jan 2010
Carbohydrates having sulfate groups such as glycosaminoglycans and chemically synthesized sucrose sulfate show interesting and important biological activities. We adapted CE with indirect UV detection technique to the determination of sulfate este...
Kakoi N, Kinoshita M, Kawasaki N, Yamaguchi T, Hayakawa T, Kakehi K
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan   129(10) 1255-1264   Oct 2009   [Refereed]
Kinoshita M, Ohta H, Higaki K, Kojima Y, Urashima T, Nakajima K, Suzuki M, Kovacs KM, Lydersen C, Hayakawa T, Kakehi K
Analytical biochemistry   388(2) 242-253   May 2009   [Refereed]
Yamada K, Kinoshita M, Hayakawa T, Nakaya S, Kakehi K
J Proteome Res.   8(2) 521-537   Feb 2009   [Refereed]
Recently, we developed an automated apparatus for rapid releasing of O-glycans from mucin-type glycoproteins and proteoglycans. In the present paper, we released O-glycans from some leukemia and epithelial cells using the apparatus, and compared t...
Matsuno YK, Kakoi N, Kinoshita M, Matsuzaki Y, Kumada J, Kakehi K
Electrophoresis. 2008 Sep;29(17):3628-35.   29(17) 3628-3635   Sep 2008   [Refereed]
Hyaluronic acid (HA) samples showing inhibition effect on digestion with testicular hyaluronidase (HAase) were found from 16 commercially available HA products, which were supplied from 11 different manufacturers. Most of these HA samples (six sam...
Ishizuka A, Hashimto Y, Naka R, Kinoshita M, Kakehi K, Seino J, Funakoshi Y, Suzuki T, Kameyama A, Narimatsu H
Biochem J.   413(2) 227-237   Jul 2008   [Refereed]
During the course of comprehensive analysis of N-glycans in cancer cell membrane fractions, we found that a significant amount of unusual, complex-type free N-glycans were accumulated in the stomach cancer-derived cell lines, MKN7 and MKN45. The m...
Taga A, Kita S, Nishiura K, Hayashi T, Kinoshita M, Sato A, Suzuki K, Kodama S, Kakehi K
J. Sep. Sci.   31(5) 853-858   Mar 2008   [Refereed]
Yamada K, Hyodo S, Matsuno YK, Kinoshita M, Maruyama SZ, Osaka YS, Casal E, Lee YC, Kakehi K
Analytical biochemistry   371(1) 52-61   Dec 2007   [Refereed]
Matsuno YK, Yamada K, Tanabe A, Kinoshita M, Maruyama SZ, Osaka YS, Masuko T, Kakehi K
Analytical biochemistry   362(2) 245-257   Mar 2007   [Refereed]
Journal of Pharmaceutical Biomedical Analysis   39(1-2) 187-195   Sep 2005
J Proteome Res.   4(1) 146-152   Jan 2005
We have to choose the most appropriate labeling method based on the purposes such as MS, HPLC and CE.In the present studies, we found a condition in which the released N-glycosylamine-type oligosaccharides were exclusively present at least during ...


Journal of Biological Chemistry   284-275 26772-26779   2000
The protease acitivoted receptor-2 agonist induces gastric mucus secretion and mucosal cytoprotection
J. Clin. Invest.   107, 1443-1450    2001

Conference Activities & Talks

Analysis of interaction between hyaluronan oligomers and hyaluronan-binding protein    Correlation between hyaluronan molecular size and binding reaction   
Mar 2003   
High-speed analysis of glycosaminoglycans by microchip electrophoresis
Dec 2003   
Size-requirement of hyaluronan for the recognition by hyaluronan-binding protein
InterLec21   May 2004   InterLec21
Previously, we reported high-resolution separation of HA using capillary electrophoresis and a buffer containing neutral polymer as sieving matrix, and found that smaller oligosaccharides than octasaccharide migrated in the reverse order of their ...
Binding analysis of alpha-galactose-binding proteins by capillary affinity electrophoresis
InterLec21   May 2004   InterLec21
In this study, following oligosaccharides were prepared enzymatically or from bird eggs to examine the binding characteristics of alpha-galactose-binding proteins: N-glycans with one to three a-Gal epitope at the nonreducing end for anti-Gal antib...
Comparative studies of glycosyltransferase activities in human cancer cells using capillary electrophoresis
Dec 2004   

Research Grants & Projects

Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(若手研究(B))
Project Year: 2009 - 2010    Investigator(s): Mitsuhiro KINOSHITA
Since structural alterations of glycans are known to be associated with changes of physiological environments, glycans have been received attention as target molecules for clinical biomarkers. In this study, we analyzed N- and O-glycans of glycopr...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2005 - 2007    Investigator(s): Kazuaki KAKEHI
In this research project, we developed a method for comprehensive analysis of N- and 0-linked glycans present on cell membrane. The method was applied to comparative analysis of total glycans expressed on various cancer cell surfaces. The total N-...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2002 - 2004    Investigator(s): Kazuaki KAKEHI
Carbohydrates as well as nucleic acids and proteins are major conctituents of glycoconjugates such as glycoproteins, glycolipids and proteoglycans. Related enzymes for the synthesis of carbohydrate chains of glycoconjugates play important roles, a...
Relationship between stereochemical structure and Biological functions
High-resolution separation of acidin glycoprotein using capillary electrophoresis