2010年9月
Induction of endosomal/lysosomal pathways in differentiating osteoblasts as revealed by combined proteomic and transcriptomic analyses
FEBS LETTERS
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- 巻
- 584
- 号
- 18
- 開始ページ
- 3969
- 終了ページ
- 3974
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.febslet.2010.07.055
- 出版者・発行元
- ELSEVIER SCIENCE BV
We have analyzed proteome changes associated with bone-forming osteoblast differentiation by quantitative differential proteomic and transcriptomic analyses using in vitro differentiation model. Sixty nine proteins were found up-regulated (>2-fold) and 18 were down-regulated (<0.5-fold) at protein level. The mRNA levels of these proteins were then analyzed by quantitative real-time PCR combined with clustering analysis. The most prominent cluster with increased protein and mRNA levels contains endosomal and lysosomal proteins, demonstrating the drastic induction of degradative endosomal/lysosomal pathways in osteoblasts. Osteoblasts, therefore, are involved not only in the synthesis but also in the turnover of the extracellular matrix proteins such as collagens. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.febslet.2010.07.055
- ISSN : 0014-5793
- PubMed ID : 20682313
- Web of Science ID : WOS:000282187500023