MISC

2003年4月1日

The regulation of bone resorption in tooth formation and eruption processes in mouse alveolar crest devoid of cathepsin K

Journal Pharmacological Sciences
  • Masayo Okaji
  • ,
  • Hideaki Sakai
  • ,
  • Eiko Sakai
  • ,
  • Mitsue Shibata
  • ,
  • Fumio Hashimoto
  • ,
  • Yasuhiro Kobayashi
  • ,
  • Noriaki Yoshida
  • ,
  • Kuniaki Okamoto
  • ,
  • Kenji Yamamoto
  • ,
  • Yuzo Kato

91
4
開始ページ
285
終了ページ
294
記述言語
英語
掲載種別
DOI
10.1254/jphs.91.285
出版者・発行元
Japanese Pharmacological Society

Osteoclastic bone resorption has recently been implicated in the tooth formation and eruption in alveolar bone. Cathepsin K (CK) is a cysteine proteinase expressed predominantly in osteoclasts and is believed to play a critical role in degradation of bone matrix proteins. Here we present evidence that the alveolar bone resorption is essential for the tooth formation and that eruption proceeds normally in CK-deficient (CK-/-) mice. Radiographic and histological analyses revealed that the alveolar bone from these animals had no significant abnormalities during the tooth development between 5 and 28 days after birth. The tooth crown was normally erupted through the alveolar bone layer at 28 days after birth. The number of tartrate-resistant acid phosphatase-positive multinuclear cells in the alveolar bone around the tooth germ was apparently increased in 5-day-old CK-/- mice compared with age-matched littermates. More important, however, the immunohistochemical localization of matrix metalloproteinase-9 (MMP-9) was clearly increased in the CK-/- osteoclasts. In contrast, no significant difference in the immunoreactivity for cathepsin D was observed between the CK-/- osteoclasts and the wild-type ones. These results indicate that CK-/- osteoclasts are fully differentiated and are capable of degrading the organic phase of alveolar bone during the tooth formation and eruption, which may result from the compensatory action by MMP-9 increasingly expressed in the osteoclasts.

リンク情報
DOI
https://doi.org/10.1254/jphs.91.285
CiNii Articles
http://ci.nii.ac.jp/naid/30003472503
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12719657
ID情報
  • DOI : 10.1254/jphs.91.285
  • ISSN : 1347-8613
  • CiNii Articles ID : 30003472503
  • PubMed ID : 12719657
  • SCOPUS ID : 12444333546

エクスポート
BibTeX RIS