2002年12月20日
Development of hydrophilic fluorogenic derivatization reagents for thiols: 4-(N-acetylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole and 4-(N-trichloroacetylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole
Journal of Chromatography A
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- 巻
- 982
- 号
- 1
- 開始ページ
- 111
- 終了ページ
- 118
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0021-9673(02)01274-8
- 出版者・発行元
- ELSEVIER SCIENCE BV
Sensitive, reactive, and hydrophilic fluorogenic reagents for thiols with the benzofurazan skeleton, 4-(N-acetylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (AcABD-F) and 4-(N-trichloroacetylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (TCAcABD-F) have been developed. These reagents reacted with thiols within 10 min at 60°C. AcABD-F and TCAcABD-F themselves do not fluoresce but are strongly fluorescent after the reaction with thiol compounds. The generated derivatives were highly water-soluble, since they dissociated a proton and ionized in the neutral pH region. The derivatives with four biologically important thiol compounds were separated on a reversed-phase HPLC column and detected fluorometrically at 504 nm with excitation at 388 nm. The detection limit attained for homocysteine with AcABD-F was 25 fmol on column (11 nM) (signal-to-noise ratio=3), and that for glutathione with TCAcABD-F was 45 fmol on column (20 nM). © 2002 Elsevier Science B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/S0021-9673(02)01274-8
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/12489860
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000179437600008&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0037146789&origin=inward
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=0037146789&origin=inward
- ID情報
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- DOI : 10.1016/S0021-9673(02)01274-8
- ISSN : 0021-9673
- PubMed ID : 12489860
- SCOPUS ID : 0037146789
- Web of Science ID : WOS:000179437600008