2018年9月
Proton Affinitive Derivatization for Highly Sensitive Determination of Testosterone and Dihydrotestosterone in Saliva Samples by LC-ESI-MS/MS
ANALYTICAL SCIENCES
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- 巻
- 34
- 号
- 9
- 開始ページ
- 1017
- 終了ページ
- 1021
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2116/analsci.18SCP08
- 出版者・発行元
- JAPAN SOC ANALYTICAL CHEMISTRY
In this study, proton affinitive derivatization using picolinic acid and its analogs (3- and 6-methylpicolinic acid and 5-butylpicolinic acid) with proton affinitive moieties was performed for the highly sensitive determination of testosterone (T) and 5 alpha-dihydrotestosterone (DHT) in saliva by LC-ESI-MS/MS. T and DHT were converted to their corresponding picolinate esters and their chromatographic behavior was investigated with a reversed phase column. The picolinate ester of each steroid exhibited a clear single peak and elution occurred in the following order: picolinate, 3/6-methylpicolinate, and 5-butylpicolinate. Estimation and understanding of the separation and retention time of each picolinate ester was made simple using the develop method. Although the peaks of picolinate and 3/6-methylpicolinate esters were suppressed by interference from the saliva background (matrix effect), the 5-butylpicolinate esters were only marginally affected.
- リンク情報
- ID情報
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- DOI : 10.2116/analsci.18SCP08
- ISSN : 0910-6340
- eISSN : 1348-2246
- Web of Science ID : WOS:000445994200006