MISC

2004年5月1日

Specific detection and identification of herpes B virus by a PCR-microplate hybridization assay

Journal of Clinical Microbiology
  • Chika Oya
  • ,
  • Yoshitsugu Ochiai
  • ,
  • Yojiro Taniuchi
  • ,
  • Takashi Takano
  • ,
  • Fukiko Ueda
  • ,
  • Yasuhiro Yoshikawa
  • ,
  • Ryo Hondo

42
5
開始ページ
1869
終了ページ
1874
DOI
10.1128/JCM.42.5.1869-1874.2004

Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.

リンク情報
DOI
https://doi.org/10.1128/JCM.42.5.1869-1874.2004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15131142
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-2442593999&origin=inward
URL
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=2442593999&origin=inward