2006年5月
Sucralfate prevents the delay of wound repair in intestinal epithelial cells by hydrogen peroxide through NF-kappa B pathway
JOURNAL OF GASTROENTEROLOGY
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- 巻
- 41
- 号
- 5
- 開始ページ
- 450
- 終了ページ
- 461
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/s00535-006-1787-0
- 出版者・発行元
- SPRINGER TOKYO
Background. Recent studies have shown that sucralfate (SF) has therapeutic effects on colonic inflammation in ulcerative colitis. The aim of this study was to clarify the function of SF for wound repair in intestinal epithelial cells (IEC). Methods. (1) Activation of signal proteins [ERK1/2 mitogen-activated protein kinase (MAPK), I kappa B-alpha] in IEC-6 cells after stimulation with 10(-4)M potassium sucrose octasulfate (SOS), which is the functional element of SF, was assessed by Western blot. (2) Induction of transforming growth factor (TGF)-beta 1, TGF-alpha, EGF, and cyclooxygenase-2 (COX-2) mRNA after stimulation of IEC-6 cells with SOS was assessed by reverse transcriptase-polymerase chain reaction. (3) IEC-6 cells were wounded and cultured for 24h with various concentrations of SOS in the absence or presence of 20 mu M H2O2. Epithelial migration or proliferation was assessed by counting migrating cells or bromodeoxyuridine (BrdU)-positive cells across the wound border. Results. (1) SOS activated I kappa B-alpha, but it did not activate ERK1/2 MAPK. (2) SOS enhanced the expression of COX-2 mRNA, but it did not change the mRNA expression of other growth factors. (3) SOS did not enhance wound repair in IEC-6 cells, but it decreased the number of dead cells (maximum, 74%) (P < 0.01) in a dose-dependent manner and prevented the diminishment of epithelial migration (maximum, 61%) (P < 0.01) and proliferation (maximum, 37%) (P < 0.05) induced by H2O2. These functions of SOS were suppressed by the NF-kappa B and COX-2 inhibitors. Conclusions. SOS prevented the delay of wound repair in IEC-6 cells induced by H2O2, probably through induction of COX-2 and an anti-apoptotic mechanism. These effects of SOS might be given through the activation of the NF-kappa B pathway.
- リンク情報
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- DOI
- https://doi.org/10.1007/s00535-006-1787-0
- CiNii Articles
- http://ci.nii.ac.jp/naid/10017615254
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/16799887
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000238547200008&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1007/s00535-006-1787-0
- ISSN : 0944-1174
- CiNii Articles ID : 10017615254
- PubMed ID : 16799887
- Web of Science ID : WOS:000238547200008