2010年5月
A simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection
GENOMICS
- ,
- ,
- 巻
- 95
- 号
- 5
- 開始ページ
- 306
- 終了ページ
- 311
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ygeno.2010.02.006
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Creating transgenic mice is an important technology for genetic studies and is currently performed by pronuclear microinjection of plasmid DNA into fertilized eggs. Since survival of injected embryos and integration of plasmid DNA are not efficient, total efficiency is only around 3% with a standard protocol. To circumvent this problem, here we describe a novel transgenesis method, the Tol2-mediated cytoplasmic injection method (Tol2:CI). We injected a foreign DNA cloned in a Tol2-transposon vector together with the transposase mRNA into the cytoplasm of fertilized eggs. As expected, the survival rate of the injected embryos was increased drastically. Also, the foreign DNA was transposed from the plasmid to the genome and transmitted to the next generation very efficiently. Together, the overall transgenic efficiency became more than 20%. Considering its simplicity and perfect compatibility with existing pronuclear microinjection facilities, we propose that the Tol2:CI method is applicable to high throughput functional genomics studies. (C) 2010 Elsevier Inc. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.ygeno.2010.02.006
- CiNii Articles
- http://ci.nii.ac.jp/naid/80021425339
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20219670
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000277258700009&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/j.ygeno.2010.02.006
- ISSN : 0888-7543
- CiNii Articles ID : 80021425339
- PubMed ID : 20219670
- Web of Science ID : WOS:000277258700009