2009年3月
A convenient method for preparation of biologically active recombinant CHH of the kuruma prawn, Marsupenaeus japonicus, using the bacterial expression system
PEPTIDES
- ,
- ,
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- 巻
- 30
- 号
- 3
- 開始ページ
- 507
- 終了ページ
- 517
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.peptides.2008.07.017
- 出版者・発行元
- ELSEVIER SCIENCE INC
Crustacean hyperglycemic hormone (CHH) not only plays an important role in the modulation of hemolymph glucose level but also functions in other biological events including molting, reproduction and stress response. Of the six CHHs characterized in Marsupenaeus japonicus, an expression system for recombinant Pej-SGP-VII (rPej-SGP-VII-amide) has not yet been established. Here, we established a procedure using a Nus-tag for solubilization, thereby soluble and biologically active rPej-SGP-VII-amide could successfully be obtained by a simpler procedure than previous ones used for producing other recombinant Pej-SGPs (Pej-SGP-I, III and IV). It was found that rPej-SGP-VII-amide thus obtained had the correct arrangement of intramolecular disulfide bonds and helix-rich secondary structure. The established expression system for rPej-SGP-VII-amide may be applicable for the preparation of other recombinant CHHs. (c) 2008 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.peptides.2008.07.017
- ISSN : 0196-9781
- Web of Science ID : WOS:000264791300009