論文

査読有り
1997年8月

The protooncogene product, PEBP2 beta/CBF beta, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures

ONCOGENE
  • Y Tanaka
  • ,
  • T Watanabe
  • ,
  • N Chiba
  • ,
  • M Niki
  • ,
  • Y Kuroiwa
  • ,
  • T Nishihira
  • ,
  • S Satomi
  • ,
  • Y Ito
  • ,
  • M Satake

15
6
開始ページ
677
終了ページ
683
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/sj.onc.1201235
出版者・発行元
STOCKTON PRESS

The Pebp62/Cbfb gene encodes the non-DNA binding beta subunit of the heterodimeric transcription factor, PEBP2/CBF, and has been implicated in a subtype of human acute myeloid leukemia, as well as being indispensable for the development of definitive hematopoiesis in the murine fetal liver, By examining a subcellular localization of the PEBP2 beta/CBF beta protein in tissue culture cells, we could reveal an additional aspect of the protein other than to be a subunit of a transcription factor, Immunoblot and immunocytochemical staining showed that PEBP2 beta/CBF beta was mostly present in the cytoplasm, This PEBP2 beta/CBF beta was free from its DNA-binding partner, the cc subunit of PEBP2/CBF, as judged by the electrophoretic mobility shift assays, Furthermore, a significant amount of PEBP2 beta 1/CBF beta was retained in the cytoskeleton preparation after detergent extraction of the cells and was found by double immunofluorescence to colocalize with the F-actin on stress fibers and the vinculin in membrane processes, Thus, the present study extends PEBP2 beta/CBF beta to be a cytoskeleton-affinitive as well as nuclear protein, The implications of these results are discussed.

リンク情報
DOI
https://doi.org/10.1038/sj.onc.1201235
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997XP68300007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/sj.onc.1201235
  • ISSN : 0950-9232
  • Web of Science ID : WOS:A1997XP68300007

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