論文

査読有り
2008年8月

Reversal of streptozotocin-induced hyperglycemia by continuous supply of betacellulin in mice

GROWTH FACTORS
  • Yoritsuna Yamamoto
  • ,
  • Satoko Yamada
  • ,
  • Tsutomu Kodera
  • ,
  • Akemi Hara
  • ,
  • Kazuo Motoyoshi
  • ,
  • Yuji Tanaka
  • ,
  • Tadahiro Nagaoka
  • ,
  • Masaharu Seno
  • ,
  • Itaru Kojima

26
4
開始ページ
173
終了ページ
179
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/08977190802136854
出版者・発行元
TAYLOR & FRANCIS LTD

Previous studies have shown the efficacy of betacellulin (BTC) to promote P-cell regeneration. Because of its short half-life, however, the effect of BTC may have been underestimated. This study was conducted to assess the effect of continuous administration of BTC on P-cell regeneration. Adenovirus vectors encoding proBTC (Ad-proBTC) and mature BTC (Ad-mBTC) were prepared, and the efficacy of secretion of BTC was compared in AML12 hepatocytes. When AML12 cells were infected with Ad-proBTC or Ad-mBTC, cells infected with Ad-mBTC secreted considerably larger amount of BTC. We then infused Ad-mBTC into the mouse tail vein. Expression of BTC was detected in the liver for at least 21 days, and serum BTC was maintained at approximately 1 ng/ml for 7 days. When Ad-mBTC was infused immediately after administration of STZ (170 mg/kg), elevation of the plasma glucose induced by STZ was markedly inhibited, and the plasma glucose concentration remained at less than 200 mg/dl for 21 days. The insulin content and the P-cell mass were significantly increased in Ad-mBTC-infused mice. These results indicate that continuous administration of BTC is quite effective in promoting regeneration of P-cells.

リンク情報
DOI
https://doi.org/10.1080/08977190802136854
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19378417
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000258769400001&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/08977190802136854
  • ISSN : 0897-7194
  • PubMed ID : 19378417
  • Web of Science ID : WOS:000258769400001

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