2004年1月
Simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by capillary zone electrophoresis
BIOMEDICAL CHROMATOGRAPHY
- ,
- ,
- ,
- ,
- 巻
- 18
- 号
- 1
- 開始ページ
- 51
- 終了ページ
- 54
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1002/bmc.291
- 出版者・発行元
- JOHN WILEY & SONS LTD
A capillary electrophoretic method for the simultaneous determination of sulfamethoxazole and trimethoprim in plasma was developed. Sulfamethoxazole and trimethoprim extracted from human plasma with ethyl acetate were analyzed at 20 kV and 25degreesC using 15 mm phosphate buffer (pH 6.2) as the electrolyte. The detection was by UV at 220 nm. The run time was 8.0 min and the limit of quantification was 10.00 mug/mL for sulfamethoxazole and 2.00 mug/mL for trimethoprim. The recovery was >99% for both compounds. This method enabled the detection of sulfamethoxazole and trimethoprim in plasma of patients after oral ingestion of their combined formulation. The present simple and rapid method is applicable to drug monitoring in immunocompromised patients who are taking the combined formulation of these compounds for the treatment or prophylaxis of Pneumocystis carinii pneumonia. Copyright (C) 2004 John Wiley Sons, Ltd.
- リンク情報
- ID情報
-
- DOI : 10.1002/bmc.291
- ISSN : 0269-3879
- Web of Science ID : WOS:000188769700009