論文

査読有り
2010年3月

Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator

SEXUAL DEVELOPMENT
  • S. Kohno
  • ,
  • Y. Katsu
  • ,
  • H. Urushitani
  • ,
  • Y. Ohta
  • ,
  • T. Iguchi
  • ,
  • L. J. Guillette

4
1-2
開始ページ
73
終了ページ
87
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1159/000260374
出版者・発行元
KARGER

Sex determination in the American alligator depends on the incubation temperature experienced during a thermo-sensitive period (TSP), although sex determination can be 'reversed' by embryonic exposure to an estrogenic compound. Thus, temperature and estrogenic signals play essential roles during temperature-dependent sex determination (TSD). The genetic basis for TSD is poorly understood, although previous studies observed that many of the genes associated with genetic sex determination (GSD) are expressed in species with TSD. Heat shock proteins (HSPs), good candidates because of their temperature-sensitive expression, have not been examined in regard to TSD but HSPs have the ability to modify steroid receptor function. A number of HSP cDNAs (HSP27, DNAJ, HSP40, HSP47, HSP60, HSP70A, HSP70B, HSP70C, HSP75, HSP90 alpha, HSP90 beta, and HSP108) as well as cold-inducible RNA binding protein (CIRBP) and HSP-binding protein (HSPBP) were cloned, and expression of their mRNA in the gonadal-adrenal-mesonephros complex (GAM) was investigated. Embryonic and neonatal GAMs exhibited mRNA for all of the HSPs examined during and after the TSP. One-month-old GAMs were separated into 3 portions (gonad, adrenal gland, and mesonephros), and sexual dimorphism in the mRNA expression of gonadal HSP27 (male > female), gonadal HSP70A (male < female), and adrenal HSP90 alpha (male > female) was observed. These findings provide new insights on TSD and suggest that further studies examining the role of HSPs during gonadal development are needed. Copyright (C) 2009 S. Karger AG, Basel

リンク情報
DOI
https://doi.org/10.1159/000260374
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19940440
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000276343400008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1159/000260374
  • ISSN : 1661-5425
  • PubMed ID : 19940440
  • Web of Science ID : WOS:000276343400008

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