The growing body of evidence of the negative impacts that roads and other linear infrastructure have on wildlife and ecosystems suggests that infrastructure represents a major driving factor of biodiversity loss. Therefore, it is important to revegetate the ground in order to prevent biodiversity loss. Arbuscular mycorrhizal fungi (AMF) colonize roots and benefit host plants by increasing growth and the ability to withstand or overcome harsh environments. In this study, we developed a revegetation filter using AMF. We mixed the host plant seeds with AMF spores and made the compound into sheets. In the sheets, the host plant roots can be infected with AMF and grow more stably and efficiently. Therefore, the revegetation filter we developed can be used in revegetation effectively.